实验性衰老小鼠肺功能和超微结构的变化及肉苁蓉多糖的影响  

The change of functions and hypermicro-instructure in lung in experimental aging mice and effect of cistanche desertica polysacchrides on the change

作　　者：孙云[1] 王德俊[1] 刘晓梅[1] 时乐 张洪泉[1] 

机构地区：[1]扬州大学医药研究所,扬州225001

出　　处：《中国药理学通报》2002年第1期84-87,共4页Chinese Pharmacological Bulletin

摘　　要：目的　观察实验性衰老小鼠肺组织细胞超微结构的退行性改变和肉苁蓉多糖对其的影响。方法　使用臭氧(Ozone,O3 )连续吸入 4wk致肺衰老模型 ,应用肉苁蓉多糖5 0 ,10 0mg·kg-1·d-1灌服模型小鼠 6wk ,检测衰老小鼠的耐缺氧能力、肺和血清中超氧化物歧化酶 (SOD)活性、丙二醛 (MDA)含量 ,电镜下观察肺组织细胞器和超微结构的变化。结果　臭氧所致实验性衰老小鼠耐缺氧能力明显下降 ;肺和血清中SOD降低、MDA升高。与模型组比较 ,肉苁蓉多糖能明显延长衰老小鼠耐缺氧时间 (P <0 0 1) ,提高血清中SOD活力、降低MDA含量 (P <0 0 1) ,延缓衰老小鼠肺细胞线粒体、板层小体等超微结构的退变 (P <0 0 1)。结论　肉苁蓉多糖能明显延缓实验性衰老小鼠肺的生理性退化与细胞形态学退变。AIM To observe the alteration of the functions and hypermicro-instructure in lung of experimental aging mice and the effect of cistanche desertica polysacchrides on the alteration. METHODS To use the models of inhaling ozone (O 3) for 4 week,and two doses of cistanche desertica polysacchrides (50,100 mg·kg -1 ·d -1 ) were ig administrated to small and large dose group mice per day respectively(for 6 week).Then the behavioral and bio-chemical examinations of lung and blood was carried out,and the ultrastructures were observe by the transmission electronic microscopic. RESULTS Compared with aging models,cistanche desertica polysacchrides could significantly prolong the period of non-oxygen ( P< 0.01),promote SOD it also activity and decrease MDA contents in lung and serum respectively ( P< 0.01), it also could improve swelling of mitochondra and the alteration of multilamellar ultrastructure. CONCLUSION Cistanche desertica polysacchrides improve the impaired physiological function and alleviate morphological change in lung in experimental aging mice.

关 键 词：肺 衰老 耐缺氧 抗氧化 超微结构 肉苁蓉多糖 肺功能 实验研究 

分 类 号：R285.5[医药卫生—中药学] R286.79[医药卫生—中医学]