双层夹心法培养大鼠Kupffer细胞  

Culturing rat Kupffer cell with double collagen culture

作　　者：王君[1] 彭仁琇[1] 夏雪雁[1] 杨静[1] 

机构地区：[1]武汉大学医学院药理学教研室,武汉430071

出　　处：《中国药理学通报》2002年第1期106-109,共4页Chinese Pharmacological Bulletin

摘　　要：目的　建立可长时间维持细胞功能 ,减少细胞破坏的Kupffer细胞培养方法。方法　细胞破坏以乳酸脱氢酶(LDH)含量反映 ,细胞功能以一氧化氮 (NO)和胞内酸性磷酸酶 (ACP)水平反映。以双层夹心法 (doublecollagenculture ,DCC)对比普通培养法 (commonculture,CC)和单层胶原培养法 (singlecollagenculture ,SCC) ,在有或无血清状态下 ,Kupffer细胞功能维持和破坏情况。 结果　双层夹心法在培养 15d内 ,能降低培养上清LDH含量 ,维持NO和胞内ACP水平 ,并可在无血清状态下减少细胞破坏 ,维持细胞功能 ,与另两种方法相比作用显著。结论　双层夹心法对体外研究Kupffer细胞具有实际应用价值。AIM To explore a long-term Kupffer cell culture method, which can best maintain the function and activity of cell and decrease the cellular damage. METHODS Lactic dehydrogenase (LDH) in the supernatants were measured to observe the severity of cellular damage ,the function of Kupffer cell was reflected with the nitrogen monoxide(NO) content and the intracellular acid phosphatases(ACP) activity. In the conditions of common culture(CC),single collagen culture(SCC) and double collagen culture(DCC), with or without serum, study the maintenance of cellular function and the severity of cellular damage. RESULTS Compared with the other two methods, the LDH contents were significantly lower( P< 0.05; P< 0.01),the levels of both NO at different days and the cellular ACP at 15d were markedly increased( P< 0.05; P< 0.01) in DCC group with serum. Even without serum, the cellular damage and function could achieve satisfactory results in DCC group. CONCLUSION DCC is a useful method to study Kupffer cell in vitro.

关 键 词：KUPFFER细胞 培养方法 普通培养法 双层夹心法 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]