HPLC法测定黄芩及其不同炮制品中黄芩苷的含量  

Measurement of Baicalin Contents by HPLC

作　　者：贺祝英[1] 曹佩雪[2] 梁光义[2] 刘再川[1] 吴兴国[1] 

机构地区：[1]贵阳中医学院第一附属医院,贵州贵阳550001 [2]中国科学院贵州省天然产物化学重点实验室,贵州贵阳550002

出　　处：《中国中药杂志》2002年第4期258-260,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目 (3 9960 0 83 )

摘　　要：目的 :测定黄芩及其不同炮制品中黄芩苷的含量。方法 :采用高效液相色谱法 ,反相C18柱 ,以甲醇 0 .0 4 %磷酸水溶液 (46∶5 4 )为流动相 ,检测波长为 2 80nm。结果 :生黄芩、酒炒黄芩、酒润麸炒法炮制的黄芩饮片中 ,黄芩苷平均含量分别为 6 .8% ,6 .0 % ,6 .7%。结论Objective:To compare the contents of baicalin in the raw medical material and the different prepared medical materials of Scutellaria baicalensis . Method:The contents of baicalin were determined by HPLC. Chromatographic conditions included Hypersil C 18 column and the mobile phase consisting of a mixture of methanol 0.04%phosphoric acid (46∶54). Baicalin was detected at 280 nm.The standard curve was linear in the range of 0.0280～4.6700 μg with correlation coefficient 1.0000. The average recovery of baicalin was 101.22% with RSD=2.54%( n =5). Result: The contents of baicalin were 6.80% in raw material, 6.00% and 6.73% in the different prepared medical materials of S. baicalensis respectively. Conclusion: The two different kinds of preparing methods can be used to the raw material of S. baicalensis .

关 键 词：黄芩 炮制 高效液相色谱法 黄芩苷 含量测定 

分 类 号：R284[医药卫生—中药学]