应用PCR-RFLP调查武汉汉族人群PGM1基因型  

PGM1 genotyping by PCR-RFLP

作　　者：宋海燕[1] 杨庆恩[1] 陈慧[1] 杨荣芝[1] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030

出　　处：《中国法医学杂志》2002年第1期17-20,共4页Chinese Journal of Forensic Medicine

摘　　要：目的PCR RFLP技术调查武汉地区汉族人群PGM 1基因型。方法应用PCR RFLP技术检测PGM 1基因型 ,调查 3 0 0例汉族无关个体。扩增PGM 1基因外显子 4和 8中的靶片段 ,并分别经过Bg1Ⅱ和NlaⅢ限制酶消化。酶切片段经聚丙烯酰胺凝胶电泳分型。结果PGM 1 RFLP技术可分出 9种基因型 ,在汉族人群 ,PGM 1 RFLP系统的个体识别能力为 0 745 0。与传统的PAGE酶型检测比较 ,本法不能区分 1+ 2 -和 1-2 +型 ,不能检测出PGM 1稀有基因 ,但克服了IEF无法分析微量、陈旧材料的缺点 ,对保存 2 5年陈旧血痕及 0 1ng模板DNA均能成功分型。 结论PGM 1Objective The purpose of this paper is to study PGM1 genotyping by PCR RFLP.Method 300 unrelated individuals of Han were genotyped using PCR RFLP. The target amplificaton products of extron 4 and 8 of PGM1 gene were digested by Bgl II and Nla III respectively.The digested DNA fragments were typed by PAGE.Result This PGM1 RFLP system can discriminate 9 genotypes with Dp of 0 7450 in Han population.Compared with conventional PAGIEF, 1+2- and 1-2+ cant be differentiated and the rare genotypes also cant be detected by this method.The advantage of this method was PGM1 genotyping successfully in bloodstains stored for 25 years and with 0 1ng genomic DNA.PGM1 RFLP method is useful for forensic identification.

关 键 词：磷酸葡萄糖变位酶-1 PGM1 PCR-RFLP 基因型 等电聚焦 法医学检测 武汉 

分 类 号：D919[医药卫生—法医学]