甲基苯丙胺及其代谢产物在急性中毒豚鼠体内的分布  

The distribution of methamphetamine and its metabolite amphetamine in guinea pig's tissues

作　　者：姜宴[1] 沈敏[2] 赵子琴[1] 陈龙[1] 叶永红[1] 向平[2] 沈保华[2] 

机构地区：[1]复旦大学医学院法医学教研室,上海200032 [2]司法部司法鉴定科学技术研究所,上海200063

出　　处：《中国法医学杂志》2002年第1期21-23,共3页Chinese Journal of Forensic Medicine

摘　　要：目的研究甲基苯丙胺 (MAP)及其代谢产物苯丙胺 (AP)在急性中毒豚鼠体内的含量分布。方法应用GC/NPD技术 ,以 4 苯基丁胺 (4 PBA)为内标 ,样品经水解后碱化或直接碱化至pH >11,环已烷混旋提取 ,三氟乙酸酐 (TFA)微波衍生化 ,测定MAP急性中毒豚鼠体液和组织中MAP及AP的含量。结果急性中毒死亡豚鼠体内各器官和体液中MAP及AP含量最高为肺 ;其次为肝、脑、肾、脾、肠、心、血 ;再次为胃、胆汁 ;最少是尿。结论MAP在动物体内代谢迅速 。Objective To study the distribution of methamphetamine(MAP) and its metabolite amphetamine(AP) in guinea pigs tissues.Method A method for the simultaneous determination of the level of the MAP and its metabolite AP in urine,blood and tissues of guinea pig was developed using gas chromatography with nitrogen phosphorus detection(GC/NPD).Samples were alkalinized to pH>11 directly or after acid hydrolysis and then were extracted by the cyclohexane,followed by derivatized with trifluoroacetic acid anhydride.4 phenylbutylamine was used as internal standard.The method was used for quantification of MAP and AP in acute poisoned guinea pigs.Results The grade of concentration of MAP and AP in acute poisoned guinea pigs is as follows:lung>liver,brain,kidney,spleen,intestine,heart,blood>stomach,bile>urine.Conclusion The metabolism of MAP in animals tissues and body fluids is related to the time elapsed after guinea pigs death.

关 键 词：甲基苯丙胺 MAP 苯丙胺 体内分布 GC/NPD 豚鼠 急性中毒 

分 类 号：R99[医药卫生—毒理学]