应用流式细胞仪研究HIV/AIDS患者的免疫状况  

Study of immunological status of HIV/AIDS patients by flow cytometer

作　　者：姚均[1] 何忠平[1] 张建平[1] 

机构地区：[1]北京地坛医院临床病毒研究室,100011

出　　处：《临床输血与检验》2002年第1期9-11,共3页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：北京市自然科学基金 ( 7982 0 2 5 )资助

摘　　要：目的　探讨人类免疫缺陷病毒 ( human immunodeficiency virus,HIV)感染者和艾滋病 ( acquired immunodefi-ciency syndrome,AIDS)患者的免疫状况。方法　应用流式细胞仪 ( flow cytometer,FCM)四色荧光计数 ( CD4 5 +、CD3+、CD4 + 、CD8+ )检测 2 0例正常人、38例 HIV无症状感染者、2 4例 HIV有症状感染者和 2 1例 AIDS患者的外周血 CD4 + 、CD8+ 淋巴细胞 ,结合临床进行分析。　结果　 HIV无症状感染者、HIV有症状感染者和 AIDS患者的外周血 CD4 + 细胞数( 45 6± 99.8、2 87± 85 .3、4 5± 4 1 .9)及 CD4 + / CD8+比值 ( 0 .4 6± 0 .1 4、0 .39± 0 .1 5、0 .1 1± 0 .0 9)均明显低于正常人群的CD4 + 细胞数 ( 84 2± 2 64.1 ) ( P<0 .0 1 )及 CD4 + / CD8+ 比值 ( 1 .79± 0 .5 1 ) ( P<0 .0 1 ) ;二者随病程进展不断下降 ,且不同病程间差异明显。结论　CD4 +细胞绝对数和 CD4 + / CD8+比值可作为检测Objective To study the immunological status of HIV/AIDS patients. Methods T cell subsets from 20 normal control subjects, 38 asympformatic HIV infected persons, 24 sympformatic HIV infected persons and 21 AIDS patients were detected by flow cytometer:four colors tru count (CD45 +,CD3 +,CD4 +,CD8 +),and the clinical stages were also analyzed. Results The numbers of CD4 + cell and the ratios of CD4 + to CD8 + in HIV infected persons and AIDS patients were less than normal control subjects significantly (P<0.01) and were decreasing with development of different infectious periods, a remarkable difference between different infectious periods was also observed. Conclusion It suggested that the numbers of CD4 + cell and the ratios of CD4 + to CD8 + might be considered as important predictors of the HIV infectious stages.

关 键 词：流式细胞仪 CD4^+细胞 CD4^+/CD8^+比值 HIV感染 AIDS T淋巴细胞 临床分期 机体免疫功能 

分 类 号：R512.91[医药卫生—内科学] R446.63[医药卫生—临床医学]