应用微卫星PCR对中国3个品系实验用小型猪的遗传检测  

Preliminary Genetic Checking on Three Strains of Chinese Laboratory Miniature Pig by Microsatellite PCR

作　　者：牛荣[1] 黄中波[1] 商海涛[1] 魏泓[1] 甘世祥[2] 王爱德[3] 曾养志[4] 

机构地区：[1]第三军医大学实验动物中心,重庆400038 [2]贵阳中医学院实验动物研究所,贵州贵阳550002 [3]广西大学动物科学技术学院,广西南宁530005 [4]云南农业大学遗传资源研究室,云南昆明650201

出　　处：《中国兽医学报》2002年第2期198-200,共3页Chinese Journal of Veterinary Science

基　　金：国家重点基础研究发展规划项目 ( G2 0 0 0 0 16 10 6 ) ;国家"九五"重点科技攻关项目 ( 96 A-23-0 6 -0 1) ;国家自然科学基金重点资助项目 ( 39830 190 ) ;国家自然科学基金资助项目( 39770 398)

摘　　要：以 12个 STR(shord tandem repeat)座位的 PCR对中国 3个品系实验用小型猪进行了遗传检测。结果 ,3个座位品系间扩增结果相同 ;9个座位呈现品系间多态性 ,其中 2个座位在 3个品系间均具有差异。贵州小型香猪与西双版纳近交系小耳猪间有 6个座位有差异 ,其遗传距离为 0 .186 4;贵州小型香猪与广西巴马小型猪间有 3个座位有差异 ,其遗传距离为 0 .12 73;广西巴马小型猪与西双版纳近交系小耳猪间有 5个座位有差异 ,其遗传距离为 0 .1333。由此表明 ,3个品系小型猪间的遗传背景具有一定差异 ,同时表明这 9个座位可选择为小型猪遗传检测的多态性座位。Porcine microsatellite DNA loci were checked by PCR on three strains of Chinese miniature pig including Guizhou miniature pig,Guangxi Bama one and Xishuangbanna inbred one.In 12 loci of three strains miniature pig,3 were monomorphic,9 were polymorphic. Two Loci could be used to distinguish the three strains of miniature pig.There were differences of 6 loci between Guizhou one and Xishuangbanna inbred one,differences of 3 loci between Guizhou one and Guangxi Bama one,differences of loci between Guangxi Bama one and Xishuangbanna inbred one.Their standard genetic distances separately are 0.186 4 , 0.127 3 , 0.133 3 .The results suggested that there were genetic variety among the three strains′ miniature pig,9 polymorphic loci could be used to distinguish the three strains′ miniature pig.

关 键 词：小型猪 微卫星RCR 遗传检测 

分 类 号：S828[农业科学—畜牧学]