宁夏滩羊体大品系OB基因的RFLP检测  

RFLP CHECKING ON BIG-BODY STRAIN'S OB GENE OF TAN SHEEP IN NINGXIA

作　　者：于洪川[1] 张振汉[1] 魏智清[1] 陈如熙[1] 杜立新[2] 龚伟宏[3] 

机构地区：[1]宁夏农学院生物系,宁夏永宁750105 [2]山东农业大学,山东泰安2710183 [3]宁夏农牧厅畜牧工作站,银川750001

出　　处：《宁夏农学院学报》2002年第1期26-27,共2页Journal of Ningxia Agricultural College

基　　金："九七"列入国家科技重点项目的内容之一;项目名称为"宁夏滩羊体大品系选育研究";项目编号97-D142

摘　　要：本研究运用了PCR -RFLPs方法 ,根据人和猪肥胖 (ob)基因序列合成的特异性引物 ,对滩羊体大品系与普通品系的基因组进行多态性分析 ,表明其扩增产物的HinfI酶切结果出现了一种T等位基因 ,而无C等位基因 。The specific primers were synthesized by PCR-RFLPs according to human and pig OB gene. The polymorphism analysis studied the genetic DNA on bigger strain and ordinary strain of Tan Sheep. The Hinf I enzym's analysis of the specific PCR products on two strains of Tan Sheep showed that OB gene locus can be detected T allece and can't be detected C allece. The OB gene locus aren't varied for two strains of Tan Sheep.

关 键 词：滩羊 RFLP OB基因 酶切 分子遗传标记 

分 类 号：S826[农业科学—畜牧学]