邻乙基苯肼盐酸盐的反相HPLC测定  

Reversed phase high performance liquid chromatographic determination of 2-ethylphenylhydrazine hydrochloride

作　　者：潘富友[1] 杨健国[1] 梁华定[1] 葛昌华[1] 

机构地区：[1]浙江省台州师范专科学校化学系,浙江台州317000

出　　处：《冶金分析》2002年第1期40-42,共3页Metallurgical Analysis

摘　　要：采用shim packCLC ODS柱 (5 μm ,2 5 0mmL× 4 6mmI D) ,乙腈 -缓冲溶液 (每升含 0 7g 1 庚磺酸钠 ,2 0mL冰乙酸 ) (3∶7,V/V)为流动相 ,检测波长为 2 5 4nm ,流速为 1mL/min ,在 5～ 15 0mg/L浓度范围内 ,反相HPLC中邻乙基苯肼盐酸盐的峰面积与浓度线性关系良好 (r=0 9999) ,RSD为 0 48% ,方法简便、准确 ,为工业化生产中动态跟踪产品质量 。The RP HPLC determination of 2 ethylphenylhydrazine hydrochloride(EPH) was carried out by using shim pack CLC ODS column(5μm,250mm L×4 6mm I D),the medium of acetonitrile:buffer(0 7g sodium 1 heptanesulfonate,2 0mL glacial acetic acid in 1000mL of water) 3∶7 (V/V) was as mobile phase,flow rate was 1 0mL/min,detection wavelength was 254nm. A good linearity was obtained in the concentration range of 5-150mg/L for EPH,The regressive equation was y=1445 81x+1335 40,r=0 9999 The method has been used to determine EPH in samples with satisfactory results

关 键 词：邻乙基苯肼盐酸盐 反相HPLC 测定 消炎镇痛药 

分 类 号：O657.7[理学—分析化学]