豚鼠心房肌细胞的分离及其电生理特性  

Isolation of guinea-pig atrial myocytes and observation of its electrophysiological properties

作　　者：孙强[1] 臧伟进[1] 于晓江[1] 赵正航[1] 王昌利[1] 

机构地区：[1]西安交通大学医学院心血管生理药理研究室,西安710061

出　　处：《西安医科大学学报》2002年第2期202-203,205,共3页Journal of Xi'an Medical University(Chinese)

基　　金：国家自然科学基金资助课题 (No .3 9870 3 3 4;No .3 9970 2 73 ) ;教育部科学技术重点项目 (0 1161);教育部高校博士学科点基金 (2 0 0 10 6980 3 4)

摘　　要：目的　建立单个心房肌细胞的急性分离方法并记录其电生理特性。方法　采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。结果　采用本方法分离出典型的豚鼠单个心房肌细胞并记录到豚鼠心房细胞毒蕈碱型钾电流及L 型钙电流。结论　此方法简单、快捷 。Objective To construct a method of isolating single guinea pig atrial myocyte and to measure its electrophysiological properties. Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.Results The typical single guinea pig atrial myocyte could be isolated with this method and muscarinic K + current (I k,ACh ) and L-type Ca 2+ current(I Ca,L ) could be recorded. Conclusion The improved method, simple and quick, can dissociate atrial myocytes with normal electrophysiological properties.

关 键 词：豚鼠 心房肌细胞 分离 电生理 膜片钳 

分 类 号：R331.3[医药卫生—人体生理学]