HPLC-DAD法测定复方心脑康软胶囊中5种有效成分含量  

Determination of five effective constituents in Compound Xinnaokang Soft Capsules by HPLC-DAD

作　　者：张虹[1] 柳正良[2] 王洪泉[1] 

机构地区：[1]同济大学附属同济医院,上海200065 [2]第二军医大学药学院,上海200433

出　　处：《中成药》2002年第4期259-262,共4页Chinese Traditional Patent Medicine

摘　　要：目的 :建立同时测定复方心脑康软胶囊中丹参酮ⅡA、丹参素、原儿茶醛、葛根素、阿魏酸 5种有效成分的含量测定方法。方法 :超声提取 ,使用C8柱 ,乙腈与水 (pH3.0磷酸调节 )按梯度洗脱程序 ,乙腈百分比于 45min内由 5 %升至72 .5 %,流速 1.5mL ,在各有效成分的最适波长下检测。结果 :各指标的平均加样回收率在 10 0 .1%～ 10 6 .9%(n =3)范围内 ,日内RSD均小于 2 .2 %(n =3)、日间RSD均小于 2 .8%(n =3) ,样品于 10h内测定稳定。结论 :本方法准确、简便、快速 ,可用于同时测定复方心脑康软胶囊中 5种有效成分的含量。Objective: To establish the method of simultaneous determination of five effective constituents tanshinose Ⅱ A、danshensu、protcate-chualdehyde、puerarin and ferulic acid. Methods: After ultrasonic extraction, HPLC-DAD were used to determine the effective constituents on Zorbax SB-C 8 column with acetonitrile and pH3.0 water(adjusted with H 3PO 4) as a mobile phase. Under the gradient timetable the percent of acetonitrile increased from 5% to 72.5% in 45 min. The flow rate was 1.5mL·min -1 , Five constituents were detected at the most suitable detection wavelength. Results: The average recovery of the five effective constituents is within the range of 100.1%～106.9%(n=3), The test of the accuracy within one day shows that the RSD is less than 2.2%, and the RSD among days less than 2.8%(n=3). The test solution is stable in 10h. Conclusion: The method is accurate, simple, quick, and can be used for determining simultaneously the content of the five effective constituents in Compound Xinnaokang Soft Capsules

关 键 词：HPLC-DAD法 测定 复方心脑康软胶囊 有效成分 含量 中药 

分 类 号：R286.0[医药卫生—中药学]