麻黄汤中麻黄碱与伪麻黄碱的GC-MS法测定及配伍因素对汤剂中该成分含量的影响  

Determination of ephedrine and pseudoephedrine in MAHUANG TANG* by GC-MS and effect of compatible medicinal herbs on concentration of components in decoction

作　　者：李吉来[1] 陈飞龙[1] 刘传明[1] 罗佳波[1] 

机构地区：[1]第一军医大学中医系,广东广州510515

出　　处：《中草药》2002年第4期307-309,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金重点资助项目( 30 0 30 150 )

摘　　要：目的　建立麻黄汤煎液中麻黄碱与伪麻黄碱的 GC-MS定量方法 ,测定麻黄汤及麻黄与其它各味中药配伍后构成的组方中麻黄碱与伪麻黄碱的煎出量 ,并考查其煎出量的变化。方法　 GC-MS选用特征离子定量法进行测定。结果　该法分离效果好 ,灵敏度高 ,重现性好。麻黄单煎液中麻黄碱与伪麻黄碱的溶出量较与杏仁合煎液中显著减少 ,较与桂枝合煎液中显著增多 ,较与甘草合煎液中无明显变化。结论　该法准确、可靠 ,可作为麻黄汤中麻黄碱与伪麻黄碱的定量方法 。Object To establish a quantitative method for ephedrine and pseudoephedrine in the aqueous decoctions of MAHUANG TANG (ADMHT) by GC MS, and to determine the content of the two components in decoctions of the different recipes consisted of Herba Ephedrae, and other herbs by using the quantitative method will be established, so as to study the effect of compatible medicinal herbs on the release of the two components in Herba Ephedrae to decoctions Methods The quantitative method by the help of characteristic ion was used in GC MS Results The content of ephedrine and pseudoephedrine in decoction prepared from Herba Ephedrae and Semen Armeniacae Amarum evidently decreases, the content of two components in decoction prepared from Herba Ephedrae and Ramulus Cinnamomin evidently increases, the content of two components in decoction prepared from Herba Ephedrae and Radix Glycyrrhizae has no obvious change, as compared with that of two components in decoction from Herba Ephedrae alone Conclusion The method is good in the aspect of separable effect, sensitivity and reproducibility The method not only can quantify the two components, but also provide the bases of quality control for the pharmacokinetic study and therapeutic detection of Herba Ephedra recipes

关 键 词：GC-MS 配伍因素 汤剂 含量 影响 麻黄汤 麻黄碱 伪麻黄碱 特征离子 中药 

分 类 号：R289.5[医药卫生—方剂学] R286.0[医药卫生—中药学]