白念珠菌基因微卫星多态性与分型探讨  

Microsatellite Polymophism of Candida albicans gene and its typing

作　　者：张军民[1] 王汉平[2] 鲁长明[1] 席丽艳[1] 曾凡钦[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院皮肤科,广州510120 [2]广州第一人民医院血液内科,510180

出　　处：《中国麻风皮肤病杂志》2002年第1期1-2,共2页China Journal of Leprosy and Skin Diseases

摘　　要：目的 :　研究白念珠菌的基因微卫星多态性特征 ,探讨新的白念珠菌的分型方法。方法 :　应用聚合酶链反应技术对 31株白念珠菌的启动子序列延伸因子 3基因的微卫星多态性作了分析。结果 :　检测到 9个等位基因 ,10种不同白念珠菌基因型 ,其基因型特征在继代培养后仍保持稳定。结论 :　该方法快捷、重复性好 。Objective: To investigate the microsatellite polymorphism of candia albicans and to create a new typing system.Methods: The polymorphism of a TTC/TTTC microsatellite in the promoter sequence of the elongation factor-3 genes in 31 strains of candida albicans was studied by polymerase chain reaction. Results:Nine alleles and 10 profiles of genes were found. This marker was stable even after successive cultures. Conclusion: As a rapid and reproducible method, the marker can be used for the study of epidemiology and genetic characterization of candida albicans.

关 键 词：白念珠菌 微卫星多态性 分型 

分 类 号：R379[医药卫生—病原生物学]