胶体金法检测梅毒特异性抗体  

Detection of specific antibody of Treponema pallidum by colloidal gold

作　　者：陈剑[1] 秦俭[1] 赵俊郁[1] 陈伟[1] 汪科[1] 朱学骏[1] 

机构地区：[1]北京大学第一医院皮肤科,100034

出　　处：《中国麻风皮肤病杂志》2002年第1期14-15,共2页China Journal of Leprosy and Skin Diseases

摘　　要：目的 :　探讨梅毒螺旋体特异性抗体胶体金法 (TPAb)对梅毒诊断的实用性和优越性。方法 :　用TPAb法对梅毒患者、非梅毒患者血清进行检测 ,同时与快速血浆反应素试验 (RPR)和梅毒螺旋体血凝试验 (TPHA)相比较。结果 :　TPAb法检测一期梅毒 2 1例敏感性为 71.4 3% ,高于RPR法 (5 7.14 % )及TPHA法 (6 6 .6 7% ) ;二期梅毒 6 4例 ,TPAb法及TPHA法检测敏感性均为 10 0 % ,高于RPR法 (92 .19% )。同时对 12 5例非梅毒患者血清进行了检测 ,结果全部阴性 ,特异性为 10 0 %。结论 :　TPAb法具有较高敏感性和特异性而且简单、方便、快速 。Objectives: To determine the applicability and superiority of TPAb assay in the diagnosis of syphilis.Methods:TPAb was used to detect the specific antibody of syphilis in serum from syphilis and non syphilis patients and compared with RPR and TPHA. Results: In 21 primary syphilis patients, the sensitivity of TPAb, TPHA and RPR was 71.43%, 66.67% and 57.14%, respectively. In 64 secondary syphilis patients, the sensitivity of TPAb, TPHA and RPR was 100%, 100% and 92.19%, respectively. In 125 non syphilis patients, the specificity of TPAb was 100%. Conclusion : TPAb is a sensitive, specific, simple and rapid method, which can be used in syphilis diagnosis.

关 键 词：胶体金法 重组TP抗原 梅毒 诊断 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]