5-Br-DMPAP选择性光度法测定发样中微量铁  

METHODOLOGY RESEARCH OF DIRECT ASSAY FOR IRON IN HUMAN HAIR WITH 5-Br-DMPAP.

作　　者：曹建明[1] 方芳[1] 周铁丽[1] 

机构地区：[1]温州医学院检验医学与公共卫生学院,温州325027

出　　处：《现代预防医学》2002年第2期180-180,192,共2页Modern Preventive Medicine

摘　　要：目的 :建立一种快速、简便、灵敏分光光度法测定发样中微量铁。方法 :在表面活性剂 Triton X- 10 0存在下 ,用 2 - (5 -溴 - 2 -吡啶偶氮 ) - 5 -二甲氨基酚 (简称 5 - Br- DMPAP)作显色剂直接光度法测定发样中微量铁。结果 :该方法在 p H4.0的乙酸 -乙酸钠缓冲液中 ,以 746 nm为测定波长 ,可选择性测定发样中微量铁 ,本法线性范围为 0～ 10 μg/ 10 m l,表现摩尔吸光数为 2 .75× 10 4L/ (mol.cm) ,回收率为 98.4%～ 10 1.3%。结论 :该法具有操作快速、简便、结果灵敏可靠等优点 。Objective:To establish a simple sensitive and stable method of determination of iron in human hair by UV-Spectrophotometry.Methods:For determination of iron in human hair with 5-Br-DMPAP in the presence of surfactant Triton X-100.Results:Iron in human hair can be selectively determined at the wavelength of 746nm in pH4.0,The linearity of test was up to 10μg/10ml.The molar absorption coefficient is 2.75×10 4L/(mol.cm).The recoveries ranged from 98.4%～101.3%.Conclusions:This method is simple,sensitive,rapid and accurate,and suitable for clinical application.

关 键 词：发样 铁 5-Br-DMPAP 分光光度法 毛发 

分 类 号：R446.19[医药卫生—诊断学] Q581[医药卫生—临床医学]