西安和郑州地区丙型肝炎患者的HCV基因分型  

Genotyping hepatitis C virus isolates from Xi'an and Zhengzhou

作　　者：霍艳英[1] 徐德忠[1] 赵小宁[1] 李如琳[1] 刘蓬勃[1] 王全楚[2] 王歆[1] 

机构地区：[1]第四军医大学预防医学系军队流行病学教研室,陕西西安710033 [2]第四军医大学唐都医院传染科

出　　处：《第四军医大学学报》2002年第8期749-751,共3页Journal of the Fourth Military Medical University

基　　金：西安市科委资助项目

摘　　要：目的　了解西安和郑州地区丙型肝炎病毒 (HCV)感染的基因型 ,比较两地基因型分布的差异 .方法　采用限制性长度多态性分析 (RFL P)法对采自西安地区的 4 6份和郑州地区的 4 0份 HCVRNA阳性标本进行 HCV基因型分型研究 .结果　西安地区 4 6份标本中 ,2 5份 (5 4 .3% )为 1b型病毒 ,17份 (37.0 % )为 2 a型病毒 ,4份 (8.7% )为 1b/ 2 a混合型病毒 ;郑州地区 4 0份标本中 ,19份 (47.5 % )为 1b型 ,13份 (32 .5 % )为 2a型 ,3份 (7.5 % )为 1a型 ,4份 (10 .0 % )为 1b/ 2 a混合型病毒 ,1份 (2 .5 % )为 1a/ 1b混合型病毒 .结论　两地的优势株均为 HCV1b型 ,其次为 2 a型 ,郑州地区有AIM To investigate the genotypes of HCV in the HCV infected samples in Xi'an and Zhengzhou. METHODS Genotypes of HCV were determined by restriction fragment length polymorphism (RFLP) analysis of the 5' untranslated regions (UTR) of different HCVs. Forty six HCVRNA positive samples from Xi'an and forty from Zhengzhou were studied. RESULTS Among the forty six samples from Xi'an: 25/46 (54.3%) were found to be of 1 b genotype, 17/46 (37.0) were 2 a genotype and 4/46 (8.7%) were mixed genotype with 1 b and 2 a. Among those from Zhengzhou: 19/40 (47.5%) were 1 b, 13/40 (32.5%) were 2 a, 3/40 (7.5%) were 1 a, 4/40 (10%) were mixed genotype with 1 b and 2 a, 1/40 (2.5%) were mixed genotype with 1 a and 1 b. CONCLUSION In both Xi'an and Zhengzhou, the predominant isolates are genotype 1 b, followed by 2 a. Cases of genotype 1 a were found in Zhengzhou.

关 键 词：西安地区 郑州地区 丙型肝炎 HCV 基因分型 

分 类 号：R512.63[医药卫生—内科学]