大剂量全身照射比格狗生物效应观察  

The biological effects of high dose total body irradiation in Beagle dogs

作　　者：罗庆良[1] 柳晓兰[1] 郝静[1] 熊国林[1] 董波[1] 赵振虎[1] 夏贞彪 邱丽玲[1] 毛秉智[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2002年第1期14-17,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：总后"九五"指令性课题资助项目 ( 96 0 5 0 0 1)

摘　　要：目的 :为极重度骨髓型急性放射病实验治疗提供依据 ,观察了不同剂量照射比格狗的生物效应。方法 :60 Coγ辐射源分别照射 6 .5 ,5 .5 ,5 .0 ,4.5 ,3.5 ,2 .5Gy ,照射剂量率为 7.2 2 4× 10 _2 C/(kg·min)。观察照射动物的一般临床表现、外周血细胞计数、骨髓细胞培养。结果 :所有比格狗于照射后 0 .5～ 2h均有呕吐。 6 .5Gy照射组动物于照射后第 2天出现腹泻 ,并伴有白色黏液 ,其中 3只动物出现血水样便或咖啡样便。 5 .5Gy组有个别动物出现水样便 ,而 4.5Gy以下剂量照射组动物出现一般稀便。比格狗除 2 .5Gy组有一只动物活存外 ,其余均死亡 ,各组死亡动物平均活存时间依剂量的大小分别为 5 .0 ,8.0 ,9.3,9.5 ,10 .5和 14.1d。照射后 1d骨髓造血祖细胞集落数随照射剂量的增加而明显减少。照射后外周血白细胞和血小板数最低值出现时间随照射剂量的增大而提前 ,且剂量效应关系显著。结论 :对临床症状、外周血白细胞及活存时间的分析结果表明 ,比格狗的极重度骨髓型急性放射病 (偏轻 )模型的全身照射剂量为 4.5～ 5 .0Gy。Objective:To evaluate the biological effects of Beagle dogs irradiated by γ_rays at different doses.Methods:All Beagle dogs were divided into six groups and were subjected respectively to total_body irradiation(TBI) with a single dose of 6.5, 5.5, 5.0, 4.5, 3,5 and 2.5?Gy γ_rays delivered by 60 Co sources at 7.224×10 _2 C/kg per minute. The general condition, blood cell counts and bone marrow cell CFC assays were observed. Results:Vomiting occurred at 0.5 to 2 hours after TBI in all groups.In 6.5?Gy group 3/5 dogs had blood_watery stool and 1/5 in 5.5?Gy group had watery stool.Diarrhea occurred in all other animals.Only one dog in 2.5?Gy group survived, all of others died.In order of decreasing irradiation dosage, the average survival time was 5.0 , 8.0 , 9.3 , 9.5 , 10.5 and 14.1 days,respectively.Conclusions:According to the clinical symptoms, leukocyte count and surviral time of the dogs, the irradiation dose which will induce very severe hematopoietic radiation syndrome in Beagle dogs is 4.5 to 5.0?Gy.

关 键 词：辐射损伤 辐射剂量 急性放射病 大剂量 全身照射 比格狗 生物效应 

分 类 号：R818[医药卫生—放射医学]