登革2型病毒PrM基因对不同型登革病毒复制的特异抑制作用  

Inhibitions of dengue 1-4 type viruses replications by the PrM gene of dengue 2 virus

作　　者：秦鄂德[1] 于曼[1] 赵月峨[1] 陈水平[1] 胡志君[1] 范宝昌[1] 段鸿元[1] 杨佩英[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《军事医学科学院院刊》2002年第1期24-27,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助项目 ( 39770 0 36 )

摘　　要：目的 :将我国登革 2型病毒PrM(D2 _PrM)基因导入甲病毒载体系统 ,探讨含正、反义PrM基因的重组病毒对不同血清型登革病毒复制的特异抑制作用。方法 :采用体外转录和电穿孔的方法 ,首先分别将构建的含正、反义PrM基因的重组质粒DNA和辅助载体DNA转录成RNA ,然后将这两种RNA共转染BHK细胞 ,进而包装成重组病毒颗粒。再将经激活的重组病毒感染宿主细胞 ,分别用不同型登革病毒攻击 ,然后通过免疫荧光法 ,观察对不同型登革病毒复制的抑制作用。结果 :含登革 2型正、反义PrM基因的重组甲病毒 ,不仅可完全抑制该型病毒在宿主细胞中的复制 ,而且对其他 3个型病毒的复制也具有不同程度的抑制作用。含反义PrM基因的重组病毒对 4个型登革病毒复制的抑制作用最强。结论 :登革 2型病毒的正、反义PrM基因在宿主细胞中通过重组甲病毒 ,可介导对登革 1～Objective:The PrM gene of dengue 2 virus was inserted into the vector system of SFV virus to observe the specific inhibitions of different type dengue viruses replications by the recombinant SFV virus contained sense_ or antisense_PrM gene of dengue 2 virus.Methods:By in vitro transcription and electroporation the recombinant plasmid DNA contained sense_or antisense_PrM gene and the helper vector DNA were transcribed into RNAs,respectively. Then these transcribed RNAs were cotransfected into the cultures of BHK cells and packaged into the recombinant virus particles. The cultures of BHK cells were infected with these activated recombinant viruses and challenged by dengue 1-4 type viruses, respectively. Then the specific inhibitions of different type dengue viruses replications were observed with immunofluorescent assay. Results:The recombinant alphaviruses containing dengue 2 sense_ or antisense_PrM gene can not only inhibit replications of this type dengue virus, but also inhibit those of other three types of dengue viruses in the cultures of BHK cells in varying degrees. The inhibition effects of the antisense_PrM gene on the replications of all four type dengue viruses were stronger than those of the sense_PrM gene.Conclusions:The sense_ or antisense_ PrM gene of dengue 2 virus can specifically inhibit replications of dengue type 1-4 viruses through the media of the recombinant alphaviruses in cultures of BHK cells.

关 键 词：甲病毒载体 抗病毒作用 登革2型病毒 PrM基因 不同型登革病毒复制 特异抑制作用 

分 类 号：R373.33[医药卫生—病原生物学]