前列腺素E_2、环磷酸腺苷在胆红素钙结石形成中的作用  

The Effect of Prostaglandin E_2 and Cyclic AMP on the Formation of Calcium Bilirubinate Gallstone Formation

作　　者：庄文[1] 肖路加[1] 左凤琼 吴兆锋 吴忠舜 

机构地区：[1]四川大学华西医院普外科 [2]四川大学华西医院基础医学院生化教研室 [3]四川大学华西医院公共卫生学院病理科,四川成都610041

出　　处：《华西医学》2002年第1期27-28,共2页West China Medical Journal

摘　　要：目的 :探讨前列腺素E2 、环酸酸腺苷在胆红素钙结石形成过程中的作用。方法 :采用肖氏兔胆红素钙结石模型进行研究 ,对照组 7只 ,胆道不全梗阻组 (BO) 36只 ,胆道不全梗阻加感染组 (BOI) 39只 ,实验组再按术后处死时间 3天、7天、14天、2 0天时相分组。动态测定各组各时相肝组织前列腺素E2 (PGE2 )、环磷酸腺苷 (cAMP)含量 ,观察肝细胞及胆汁糖蛋白变化情况。结果 :实验组肝组织前列腺素E2 、cAMP及肝细胞糖蛋白均较对照组升高有差异(P <0 0 5 ) ,胆汁糖蛋白增多。BOI组上述改变明显于BO组有差异 (P <0 0 5 ) ,PGE2 升高与cAMP升高呈正相关 (P<0 0 5 )。结论 :胆红素钙结石形成过程中存在肝组织PGE2 升高通过cAMP表达增强引起肝细胞糖蛋白合成增多促进成石这一途径。细菌感染可能通过产生更多的PGE2 ,而使结石形成增多。Objective:To investigate the effect of prostaglandin E 2 and cyclic AMP on the process of calcium bilirubinate gallst one formation.Methods:The research adopted Xiao's rabbit models.E ig hty-two rabbits were randomly divided into the control group(Con,N=7),simple bil iary obstruction group(BO,N=36)and billiary obstruction with infection group(BOI , N=39).In experimental groups the prostaglandin E 2(PGE 2)and cyclic AMP(c AMP)of liver tissue were measured and the glycoprotein changes of hepatocytes and bi le were observed in 3 rd?7 th?14 th and 20th day.Results:There existed continuously increasing of PGE 2?cAMP in liver tissue and glycoprotein in hepatocytes of experimental groups.The glycoprotein increased in bile.Ther e were apparen differences as compare with the control group( P <0 05).The cha nges of BOI group were more marked as compare with BO group and there was diffe rence between BOI and BO group( P <0 05).There was a positive corelation b etween PGE 2 and cAMP( P <0 05).Conclusions:There may be existed a route to the formation of calcium bilirubinate gallstone formation:PGE 2 to cAMP to glycoprotein to stone.Bacterial infection might result PGE 2 production mo re,and promole more stone formation.

关 键 词：兔模型 胆红素钙结石 前列腺素E2 环磷酸腺苷 糖蛋白 

分 类 号：R657.4[医药卫生—外科学]