乌鲁木齐市维吾尔族人群NA等位基因频率调查分析  

The neutrophils antigen gene allele frequency of Uygur people in Urumqi region

作　　者：李林[1] 高博[2] 关亚群[1] 王丽容[1] 赵学信[1] 

机构地区：[1]新疆医科大学基础医学院生物化学教研室,新疆乌鲁木齐830054 [2]乌鲁木齐市血液中心,新疆乌鲁木齐830000

出　　处：《新疆医科大学学报》2002年第1期35-36,共2页Journal of Xinjiang Medical University

摘　　要：目的 :通过中性粒细胞抗原 (NA)等位基因的频率调查 ,了解乌鲁木齐市维吾尔族人群 NA基因的多态性 ,为 NA相关的输血性疾病建立可靠的基因诊断技术。方法 :优化并建立了多重 PCR- SSP法 ,对 12 0名健康、无血缘关系的乌鲁木齐维吾尔族个体进行中性粒细胞抗原 (NA)的 NA1 和 NA2 种等位基因频率的调查。结果 :该样本中 NA1 基因频率为 0 .383;NA2 基因频率为 0 .6 17。结论:多重 PCR- SSP法分析 NA基因频率结果较为可靠 ,重复性较好。Objective: To study the neutrophils antigen gene allele frequency of Uygur people in Urumqi region for gene diagnosis of some transfusion diseases. Methods: A optimum multiprimered PCR-SSP method was established to screen the netrophils antigen (NA) genotype of DNA samples isolated from 120 Uygur people blood donors in Urumqi region. Results: The allele frequency of NA-1 was 0.383 and NA-2 was 0.617 in this sample volume. Conclusion: The multiprimered PCR-SSP method is confirmed to be reliable and repeatable in our experiments for NA allele typing.

关 键 词：中性粒细胞抗原 基因频率 PCR-SSP 维吾尔族 

分 类 号：Q987[生物学—遗传学]