喘宁软胶囊对嗜酸性粒细胞和肥大细胞的抑制作用  

Inhibitory effect of CHUANNING SOFT CAPSULE on eosinophils and mast cells

作　　者：金赛红[1] 谢强敏[1] 沈文会[1] 陈季强[1] 赵余庆[2] 徐有信 

机构地区：[1]浙江大学医学院呼吸药物研究重点实验室,浙江杭州310031 [2]沈阳东宇天然药物研究院,辽宁沈阳110015

出　　处：《中草药》2002年第3期242-244,共3页Chinese Traditional and Herbal Drugs

基　　金：国家重点科技(攻关)项目 (96-90 1-0 5 -2 13)

摘　　要：目的　初步探讨喘宁软胶囊的平喘作用机制。方法　采用整体试验观察致敏小鼠抗原攻击后肺灌洗液和周围血中的嗜酸性粒细胞聚集反应 ,采用离体试验观察致敏大鼠抗原攻击后腹腔肥大细胞脱颗粒反应。结果　喘宁软胶囊 2 ,4 ,8g/ kg和对照药地塞米松 2 m g/ kg ig给药显著减少肺灌洗液和周围血中嗜酸性粒细胞的浸润。喘宁软胶囊 33.3,6 6 .7mg/ L 和对照药酮替芬 0 .333m g/ L 明显抑制致敏大鼠腹腔肥大细胞脱颗粒反应。Object To explore the antiasthma mechanism of CHUANNING SOFT CAPSULE (CNSC). Methods The eosinophils accumulation in bronchoalveolar lavage fluid (BALF) and peripheral blood in the ovalbumin sensitized mice after challenged with antigen in vivo was examined and the granulation of the peritoneal mast cells in the ovalbumin sensitized rats after challenge of antigen in vitro was observed. Results CNSC 2, 4, 8 g/kg (ig) and dexamethasone 2 mg/kg (ig) significantly reduced the eosinophils infiltration in BALF and peripheral blood. CNSC 33.3, 66.7 mg/L and ketotifen 0.333 mg/L significantly inhibited granulation of mast cells. Conclusion The antiasthma mechanism of CNSC probably correlates with suppressing eosinophils infiltration in lung and stabilizing mast cell membranes.

关 键 词：喘宁软胶囊 酮替芬 地塞米松 嗜酸性粒细胞 肥大细胞 离体实验 

分 类 号：R285.5[医药卫生—中药学]