普通棉耳狨猴中乙型流感病毒的分离和鉴定  

ISOLATION AND IDENTIFICATION OF INFLUENZA B VIRUS FROM COMMON COTTON-EARED MARMOSETS

作　　者：石建党[1] 李晓眠[1] 刘凤勇[2] 金孟珏[2] 刘民[1] 徐燕[1] 张国际[1] 何建民[1] 

机构地区：[1]天津医科大学微生物学教研室,天津300070 [2]天津医科大学实验动物中心,天津300070

出　　处：《南开大学学报（自然科学版）》2002年第1期12-15,共4页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：乙型流感病毒是引起人类局部流行性感冒的重要病原体 ,其起源和自然储存宿主目前仍不清楚 .1 999年夏季在某动物中心饲养的普通棉耳狨猴 ( Callithrix jacchus)群体中爆发了以呼吸道症状为主的急性传染病 ,死亡比率高达 1 /3.通过对死亡狨猴肺组织匀浆接种鸡胚和 MDCK细胞的分离培养 ,分离出病毒 .双份血清的红细胞凝集抑制试验证实 ,此分离株为此次狨猴群体感染流行的病原体 .通过甲型和乙型流感病毒标准血清鉴定 ,证实该分离株为乙型流感病毒 ,命名为 B/marmoset/China/1Influenza B virus is a fearful human pathogen whose origin and possible reservoir host in nature are not know. An acute infectious disease broke out among the common cottoneared marmosets bred in an experimental animal center in the summer, 1999. The mortality was up to 1/3. A virus was isolated from the lung tissue of dead marmosets, which was confirmed to be the pathogen of the disease this time by the hemagglutination inhibition (HI) test with double sera. Serology analyses with standard sera of influenza A and B virus indicated that the pathogen is an influenza B virus. The virus is named as B/marmoset/China/1/99.

关 键 词：普通棉耳绒猴 乙型流感病毒 红细胞凝集抑制试验 病毒分离 病毒鉴定 病原体 

分 类 号：S858.93[农业科学—临床兽医学] S852.65[农业科学—兽医学]