胸腺肽降低环磷酰胺诱导的小鼠骨髓嗜多染红细胞微核率的初步研究  

Thymosin decreases the frequencies of micronucleated polychromatic erythrocytes of bone marrow in mice induced by cyclophosphamide

作　　者：杨录军[1] 田荣福[1] 周利军[1] 黄建[1] 曹佳[1] 

机构地区：[1]第三军医大学预防医学系分子毒理学实验室,重庆400038

出　　处：《第三军医大学学报》2002年第4期416-417,共2页Journal of Third Military Medical University

摘　　要：目的　探讨胸腺肽对遗传损伤的作用。方法　以每天 2 5 0mg kg(高剂量组 )、12 5mg kg(低剂量组 )的胸腺肽连续 10d小鼠腹腔注射给药 ,实验另设以生理盐水 10ml kg代替胸腺肽的阴性对照组 ;于第 9、10两天以 40mg kg剂量腹腔注射染色体断裂剂环磷酰胺 ,环磷酰胺末次注射后 6h处死小鼠 ,检测小鼠骨髓嗜多染红细胞微核率。结果　胸腺肽能显著降低环磷酰胺诱导的微核率 ,与阴性对照组相比P均小于 0 0 1,高低两个胸腺肽剂量组及阴性对照组的微核率分别为 (12 5 0± 3 88)‰、(14 75± 3 42 )‰ ,(2 2 5 8± 4 98)‰。结论　胸腺肽具有拮抗或修复环磷酰胺诱导的遗传损伤作用。Objective To explore the effects of thymosin on the genetic damage since it has been wildly used in clinic. Methods Thymosin at 25.0 mg/kg (high dose group) or 12.5 mg/kg (low dose group) was injected intraperitoneally to mice for 10 d in order to stimulate the immune system. Cyclophosphamide which induces micronuclei was given in same way on the 9th and 10th day. The micronucleated polychromatic erythrocytes of bone marrow were observed and measured. Results Thymosin significantly decreased the frequencies of the micronucleated polychromatic erythrocytes induced by cyclophosphamide ( P <0.01 vs negative control group). The frequencies of the high dose group, low dose group and negative control group were (12.50±3.88)‰,(14.75±3.42)‰,(22.58±4.98)‰, respectively. Conclusion Thymosin can antagonize or/and repair the genetic damage induced by cyclophosphamide.

关 键 词：胸腺肽 微核 突变 环磷酰胺 小鼠 骨髓 嗜多染红细胞微核率 

分 类 号：R979.5[医药卫生—药品]