保存人红细胞的新策略—抗氧化剂保养液保存效果的研究  

New Tactics of Human Red Blood Cells Stored at 4℃—Protective Effect of Antioxidant Solution on Red Blood Cells Damage

作　　者：马恩普[1] 刘秀珍[1] 韩颖[1] 刘明东[1] 任素萍[1] 刘安[1] 靳鹏[1] 卜凤荣[1] 吴祖泽 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《中国实验血液学杂志》2002年第2期153-155,共3页Journal of Experimental Hematology

基　　金：全军医药卫生指令性科研课题基金资助编号 960 50 0 6

摘　　要：塑料血袋盛义务献血员的静脉血 (含适量保养液 )并置 4℃冰箱保存。在保存前、后不同时间检查各项指标 ,旨在研究抗氧化剂保养液延长人血红细胞在 4℃条件下的保存时间 ,以缓解血液保存供应的压力 ,为输血抢救创造有利条件。实验分 3组 :ACD组 ,GMA组和抗氧化剂 (SOD)组。研究结果表明 ,在 4℃条件下保存 75天后SOD组红细胞血红蛋白回收率为 87.2 % ,血浆血红蛋白 (mg/L)为 193.2 ,P5 0 (mmHg)为 34.0 (正常值为 33.1) ,最大变形指数 (DImax)为 0 .2 4 13,即为正常值的 74 .3% ,外观检查无明显溶血、变色、气泡和凝块。结论提示 ,用抗氧化剂保养液保存红细胞在 4℃条件下可延长到 75天 ,其红细胞血红蛋白和血浆血红蛋白回收率附合国家《血站基本标准》Aliquots of venous blood from healthy donor were collected in plastic blood storage bags with ACD, GMA or antioxidant solution(superoxide dismutase, SOD), respectively, and stored at 4℃. After storage for varying periods, the parameters of the blood were detected in the blood samples. Results showed that the parameters of the blood stored at 4℃ for 75 days in SOD group were following: the recovery of RBC Hb was 87.2%, plasma Hb(mg/L) was 193.2, P50(mmHg) was 34.0 (normal value was 33.1); deformability(DImax) was 0.2413 (74.3% of normal value). There was no evident hemolysis, color change, air bubble and clots. It was concluded that human RBC stored at 4℃ for 75 days with SOD solution, recovery of levels of RBC Hb and plasma Hb were accorded with the requirements of 《Basic Demands of Blood Station》 in China.

关 键 词：血液保存 红细胞 超氧化物歧化酶 抗氧化剂保养液 

分 类 号：R457.12[医药卫生—治疗学]