汉滩型与汉城型汉坦病毒基因重排的初步研究  

A preliminary genetic reassortment between Hantaan virus and Seoul virus strains

作　　者：康文臻[1] 黄长形[1] 白雪帆[1] 杨为松[1] 李光玉[1] 

机构地区：[1]第四军医大学唐都医院感染病科,西安710038

出　　处：《中华流行病学杂志》2002年第1期46-49,共4页Chinese Journal of Epidemiology

基　　金：国家自然科学基金资助项目 ( 396 70 6 5 0 )

摘　　要：目的　探讨汉坦病毒汉滩型与汉城型之间能否发生基因重排以及发生重排的频率和特点。方法　用汉坦病毒汉滩型 76 118株与汉城型SR 11株混合感染VeroE6细胞 ,空斑形成试验挑取子代病毒克隆株 ,分型聚合酶链反应方法鉴定子代病毒基因型。结果　大部分 (6 8.19%)子代病毒的基因组来自 7 118株或SR 11株 ;2株 (4 .5 5 %)两型引物扩增均为阳性 ;2株 (4 .45 %)两型引物扩增均为阴性 ;5株 (11.36 %)发生了M片段重排 ,3株 (6 .82 %)发生了L片段重排 ,未发现S片段重排 ;1株M片段来自双亲株 ,1株S片段为双倍体。结论　证实汉坦病毒Ⅰ型与Ⅱ型之间可以发生重排。Objective To determine the frequency and characteristics of reassortment among Hantaan and Seoul viruses causing hemorrhagic fever with renal syndrome (HFRS). Methods Mixed infections were initiated in tissue culture, using Hantaan virus strain 76-118 and Seoul virus strain SR-11. Potential reassortant virus plaques were picked out by multiplex RT-PCR, using primers specific for individual genome segments(L,M,S) of each strain. Results Most of the progeny virus plaques ( 68.19% of 44) had parental genotype of 76-118 strain or SR-11 strain while 2 of 44 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 68.19% of 44 progeny plaques tested, involving the M and S segments. In addition, approximately 4.55% of the progeny virus plaques appeared to contain S or M segments originating from both parental virus strains, showing that they were diploid. Conclusion Genetic reassortment can occur between Hantaan virus and Seoul virus strains.

关 键 词：汉坦病毒 流行性出血热 基因重排 聚合酶莲反应 

分 类 号：R373[医药卫生—病原生物学]