陕西人群HUMARA基因多态与衰老的相关性  

Relationship between HUMARA gene polymorphism and aging in Shaanxi population of China

作　　者：冯蕾[1] 舒青[1] 秦鸿雁[2] 刘军[3] 李军林[1] 

机构地区：[1]第四军医大学基础部遗传学与发育生物学教研室,陕西西安710033 [2]1第四军医大学基础部遗传学与发育生物学教研室,陕西西安710033 [3]第四军医大学唐都医院心血管内科,陕西西安710038

出　　处：《第四军医大学学报》2002年第5期409-411,共3页Journal of the Fourth Military Medical University

摘　　要：目的　探讨陕西人群 HUMARA基因多态性分布及与衰老的相关性 .方法　应用聚合酶链反应 (PCR- STR)技术先后对老年人 (92例 )和青年人 (6 3例 )的 HU MARA基因的多态位点进行检测 .结果　在老年组中 ,片段大小在 2 80～4 4 5 bp之间 ,重复片段为 (GCA) ,重复次数最少 2 2次最多 77次 .频率分布在 0 .0 0 5～ 0 .39,高峰在 30 0～ 310 bp(39% ) ,杂合率为 0 .83,多态信息量 (PIC)为 0 .76 ;在青年组中 ,片段大小在 2 80～ 4 0 8bp之间 ,重复次数最少 2 2次最多 6 5次 ,频率分布在 0 .0 1～ 0 .33,高峰在 310～ 32 0 bp(33% ) ,杂合率为 0 .90 ,多态信息量 (PIC)为 0 .83.老年组基因频率分布与青年组比较 ,有显著性差异 (P<0 .0 1) .结论　推测该位点遗传多态与细胞的衰老有一定的相关性 。AIM To investigate the polymorphism of the HUMARA gene in Shaanxi population of China and its relationship to aging. METHODS Polymorphism sites of the HUMARA gene in 92 cases of unrelated aged people and 63 cases of unrelated youth in Shaanxi were detected by PCR STRmethod. RESULTS The allele fragments ranged from 280 to 445 bp in the aged. The repetitive fragment was (GCA) and repetitive fraguency was 22 to 77. The distribution of allele frequency was 0.005 to 0.39. The heterozygosity rate was 0.83 and polymorphism information content (PIC) was 0.76. The allele fragments ranged from 280 to 408 bp in the youth. The repeat number was 22 to 65. The distribution of allele frequency was 0.01 to 0.33. The heterozygosity rate was 0.90 and PIC was 0.83. The distribution of polymorphism of the HUMARA gene varied significantly between the aged and the youth ( P <0.01). CONCLUSION The relationship between the HUMARA gene and aging needs further study.

关 键 词：陕西人群 HUMARA基因 遗传多态 衰老 

分 类 号：R339.38[医药卫生—人体生理学]