cDNA-AFLP技术在植物差异基因表达研究上的应用  

cDNA- AFLP and Its Application in Study about Differences in Gene Expressions Analysis of Plants

作　　者：尚雨丝[1,2] 罗蕊[1,2] 王文跃[1,2] 王超[1,2] 陈永胜[1,2] 

机构地区：[1]内蒙古民族大学农学院,内蒙古通辽028043 [2]内蒙古自治区高校蓖麻产业技术研究中心,内蒙古通辽028043

出　　处：《内蒙古民族大学学报（自然科学版）》2014年第3期319-322,共4页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：国家自然科学基金项目(31160290);教育部新世纪优秀人才支持计划(NCET-08-0870);内蒙古民族大学研究生科研资助项目(S10136201308);市校合作项目(SXZD2012006)

摘　　要：cDNA-AFLP技术是能够对生物体转录组进行全面分析的新技术,因其具有多态性丰富、稳定性高、效率高等优点,被广泛应用于基因表达研究.随着科技发展,该技术不断得到完善与创新,并取得了很多研究成果.本文简单介绍了cDNA-AFLP技术的基本原理及技术特点,概述了其在植物差异基因表达上的应用现状,并对其发展前景进行了展望.cDNA-AFLP technique is a new technology to conduct a comprehensive analysis of an organism＇s transcriptome. With the advantages of rich polymorphism, high stability, high efficiency, it has been widely used to study gene expression. With advances in science, the technology has been continuously improved and innovation, and made a lot of research results. The paper introduces the basic principles and the technical characteristics of cDNA-AFLP and outlines the application of the status on differences in gene expressions of plants. And its development prospects were discussed.

关 键 词：差异基因表达 

分 类 号：Q786[生物学—分子生物学]