bFGF对缺氧复氧体外骨骼肌细胞Bc1-2表达的影响  

Effect of bFGF on Bcl-2 expression in anoxia and reoxygenation injured cultured skeletomyocytes

作　　者：马焰[1] 孙月芬[1] 高伟[1] 周长满[2] 

机构地区：[1]北京军医学院内科教研室,北京100071 [2]北京军医学院神经生物中心,北京100071

出　　处：《第四军医大学学报》2002年第6期524-526,共3页Journal of the Fourth Military Medical University

基　　金："九五"军队医药卫生科研基金课题 ( 98M118)

摘　　要：目的　观察碱性成纤维细胞生长因子 (b FGF)对缺氧复氧 (A/ R)损伤后肌细胞凋亡 (apoptosis)相关基因蛋白Bc1- 2表达的影响 .方法　将培养的骨骼肌细胞分为正常对照组、A/ R组及浓度分别为 10 ,2 0和 40μg· L- 1 的 b FGF预处理加 A/ R组共 5组 ,然后用免疫组化方法 (ABC法 )进行染色 ,统计分析 Bc1- 2阳性的肌细胞百分率、平均吸光度的变化 .结果　经 b FGF预处理后 ,Bc1- 2阳性的肌细胞百分率(如 b FGF2 0 μg· L- 1组与对照组相比 ,2 3.3%→ 34 .7% ,P<0 .0 1)、平均吸光度 (如 b FGF 2 0μg· L- 1 组与对照组相比 ,0 .13→ 0 .2 0 ,P<0 .0 1)均显著增加 .结论　 b FGF对 A/AIM To observe the Bcl-2 expression on the cultured skeletal muscle cells in neonatal rats injuried by anoxia/ reoxygenation (A/R) and preconditioned by basic fibroblast growth factor (bFGF). METHODS We divided cultured skeletomyocytes into five groups, including control group, anoxia/reoxygenation(A/R) group, bFGF preconditioned with A/R groups (bFGF density was 10, 20, 40 μg·L -1 re-spectively). Then we used immunohistochemical method (ABC method) to dye culture skeletomyocytes. RESULTS Being preconditioned by bFGF, Bcl-2 positive skeletom-yocytes' percentage rose (such as bFGF 20 μg·L -1 group compared to control group, 23.3%→34.7%, P< 0.01) and average light density increased (such as bFGF 20 μg·L -1 group compared to control group, 0.13→0.20, P< 0.01). CONCLUSION bFGF probably inhibits the occurrence of apoptosis of cultured skeletomyocytes injuried by A/R.

关 键 词：成纤维细胞生长因子 碱性 肌 骨骼肌细胞 细胞学 细胞低氧 基因 Bcl-2 

分 类 号：R337[医药卫生—人体生理学]