昌台牦牛乳酸脱氢酶-b基因2个突变位点的检测  

Detection of Two Mutations in Lactate Dehydrogenase B Gene of Changtai Yak

作　　者：许雪芬[1] 高杰[1] 蒋忠荣 刘曦 赵永华 彭海云 泽丹多吉 郑玉才[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]甘孜州畜牧科学研究所,四川康定626000 [3]甘孜州昌台种畜场,四川白玉627100

出　　处：《西南农业学报》2014年第3期1284-1287,共4页Southwest China Journal of Agricultural Sciences

基　　金：农业部行业专项(201203010);西南民族大学研究生创新型科研项目(CX2013SZ60)

摘　　要：本研究分析了昌台牦牛(Bos grunniens)乳酸脱氢酶-1(LDH1)的遗传多态性。实验提取了80头昌台牦牛基因组DNA,采用PCR-RFLP和新建立的等位基因特异PCR技术,分别对LDH1的H亚基编码基因ldhb的2个突变点进行检测。结果表明,昌台牦牛中检测到2种ldhb G896A基因型(AA、AG),未检测到GG型,其中形成LDH1快带的G等位基因频率为0.1125,且均为杂合型(AG);首次从基因水平上分析牦牛ldhb A689C基因型,观察到AA和AC共2种基因型,其中仅1头牦牛携带LDH1慢带相关的C等位基因,且为AC杂合型。试验结果表明,昌台牦牛ldhb存在与其他牦牛品种类似的多态性;新建立的等位基因特异PCR引物扩增方法能准确检测牦牛中突变频率很低的ldhb A689C。In this paper,the genetic polymorphism of lactate dehydrogenase-1 （LDH1） of Changtai yak （Bos grunniens） was analyzed.Genommic DNA was extracted from 80 Changtai yaks and used to detect two mutations in Changtai yak ldhb gene encoding the H subunit of LDH1 by using a PCR-RFLP protocol and a newly developed allele-specific PCR method.The result showed that two genotypes （AA and AG） of ldhb were detected in Changtai yaks,excluding GG genotype.The frequency of LDH1-F related G allele was 0.1125,all showing AG genotype.The genotype of ldhb A689C was assayed at gene level for the first time,two genotypes （AA and AC） were observed,and only one yak carried LDH1-S related C allele,with genotype of AC.The present results indicated that the polymorphism of Changtai yak ldhb was similar to those of other yak breeds.The newly established allele-specific method was effective to detect the ldhb A689C mutation which occurred in yaks with very low frequency.

关 键 词：牦牛 乳酸脱氢酶 遗传多态性 

分 类 号：S858.23[农业科学—临床兽医学]