天目铁木丛生芽诱导和试管苗保存技术  

A technique on shoots induction and profitability in vitro conservation with plantlets of Ostrya rehderiana

作　　者：顾地周[1] 沈红梅[1] 闫中雪 徐鹏佳 袁浩[1] 

机构地区：[1]通化师范学院生命科学学院,吉林通化134002

出　　处：《林业科技开发》2014年第4期79-82,共4页China Forestry Science and Technology

基　　金：国家科技部"国家科技攻关计划引导项目(编号:2005BA741C);吉林省科技厅资助项目(编号:200705C05)

摘　　要：以天目铁木嫩茎为外植体,应用均匀设计法筛选其基部直接再生丛生芽苗和试管苗保存的最适合培养基。结果表明,最适合的嫩茎尖基部再生芽苗培养基为:1/4 DR+TDZ 3.80 mg/L+NAA 0.03 mg/L+KT 1.50mg/L,诱导率达99.0%以上;试管苗保存培养基为:1/8 DR+TDZ 0.45 mg/L+KT 1.60 mg/L+根皮苷2.80 mg/L,在试管内保存18个月,平均生长率为2.26%。成功建立了天目铁木嫩茎基部直接再生丛生芽苗和试管苗保存体系。In this study, the tender stem of Ostrya rehderiana was used as explant and the suitable medium compositions were screened for multiple shoots at base of tender stem and in vitro conservation of plantlets by using uniform design experiments. The results showed that the most suitable culture mediums were as following： 1/4 DR ＋ TDZ 3.80 mg/L ＋ NAA 0.03 mg/L ＋ KT i. 50 mg/L for bud seedling regeneration at base of tender stem, the rate of induction was 99.0% ; 1/8 DR ＋ TDZ 0.45 mg/L ＋ KT 1.60 mg/L ＋ Phloridzin 2.80 rag/L, the rate of average growth was 2.26% with 18 month in vitro conservation. Multiple shoots at base of tender stem and in vitro conservation of plantlets of O. rehderiana had been successfully established.

关 键 词：天目铁木 丛生芽 试管苗 保存 均匀设计 

分 类 号：S531.04[农业科学—作物学]