柠檬叶挥发性成分的提取及分析  

Extraction and determination of volatile constituents in leaves of Citrus limon

作　　者：周丽珠[1] 李军集[1] 梁忠云[1,2] 文彩琳[1] 

机构地区：[1]广西林业科学研究院,南宁530002 [2]国家林业局中南速生材繁育实验室

出　　处：《林业科技开发》2014年第4期99-101,共3页China Forestry Science and Technology

基　　金：广西财政专项基本科研业务费(编号:林科201407号);广西林业厅项目(编号:桂林科字[2006]第16号)

摘　　要：采用水蒸气蒸馏法从柠檬叶中提取挥发油中的油相成分,进一步以乙醚为溶剂从蒸馏馏出液中萃取挥发油中的水溶性物质;利用气相色谱-质谱联用(GC-MS)表征了二者的化学成分,根据气相色谱峰面积归一化法进行定量。通过表征和分析比较得到:柠檬叶挥发油油相成分得率为0.33%(以鲜叶计),确定了其中的30种成分,占油相成分总量的96.35%,其主要成分为d-柠檬烯(29.91%),其后依次为β-蒎烯(20.37%)、香茅醛(18.52%)、桧烯(5.74%)。柠檬叶水溶性成分得率为0.03%(以鲜叶计),确定了其中的30种成分,占水相成分总量的97.94%,其主要成分为d-柠檬烯(30.33%)、香茅醛(21.88%)、β-蒎烯(19.83%)、桧烯(6.49%)。The volatile constituents in leaves of Citrus limon,included oil fraction and water-siluble fraction. 0il fraction of volatile components was obtained through steam distillation. Ether was used as the solvent to extract the water-siluble fraction of volatile compounds in order to know quantity and constituents of volatile compounds dissolved in the water phase. The oil yield in the oil fraction was 0.33%, and the oil yield in the water-siluble fraction was 0.03% （both on fresh weigh basis）. Both oil fraction and water-siluble fraction were quantitatively and qualitatively analyzed by means of GC-MS and GC. The results showed that both 30 compounds （96.35%）in oil fraction and （97.94 % ）water-siluble fraction were identified. The major constituents in oil fraction were d-limonene （29.91%） , followed by β-pinene （20.37%） , citronellal （18.52%） , sabinene（5.74% ）. The major constituents in water-siluble fraction were d-limonene （30.33%） ,β-pinene （ 19.83 % ）, citronellal （ 21.88 % ）, sabinene （ 6.49 % ）. In conclusion the comprehensive utilization value of leaves C. limon was enhanced owing to extraction of water-siluble volatile components.

关 键 词：柠檬叶 挥发油 水溶性成分 d-柠檬烯 GC—MS 

分 类 号：TQ654.2[化学工程—精细化工]