益气活血方药抗大鼠肝纤维化作用的实验研究  

Study of Effects and Mechanisms against Experimental Hepatic Fibrosis in Rats with Chinese Medicine

作　　者：王健[1] 河福金[1] 牛建昭[1] 郭顺根[1] 王继峰[1] 于世瀛[1] 

机构地区：[1]北京中医药大学细胞与生化实验室,100029

出　　处：《中西医结合肝病杂志》2002年第2期86-88,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家中医药管理局中医药科学技术研究基金(2000-J-P-20;2000-J-P-31)

摘　　要：目的:研究益气活血方药抗大鼠实验性肝纤维化的效应及作用机制。方法:60只雄性Wistar大鼠被分为6组(正常对照组、阴性药对照组、阳性药对照组、大剂量组、中剂量组、小剂量组),猪血清腹腔注射复制动物模型,用益气活血药物治疗7W后,处死动物,取材。Mallory染色,经图像分析仪处理,观察肝脏胶原纤维生成量。分离鼠肝星状细胞(HSC),体外培养,用内毒素(lipopolysaccharide,LPS)刺激其增殖,MTT法测定细胞增殖及含药血清对其增殖的抑制作用。结果:益气活血药物有明显的抗肝纤维化作用。小剂量组肝胶原纤维量明显低于阴性药对照组(P<0.01),与正常对照组比较无显著性差异(P>0.05)。含药血清明显抑制LPS激活的体外培养的鼠HSC增殖(P<0.01)。结论:益气活血方药具有明显抗实验性肝纤维化的效应,其作用机制之一是抑制HSC的增殖。Objective: To study the effects and mechanisms against experimental hepatic fibrosis with prescription for benefited Qi to promote flow of blood. Methods: Hog serum was injected into abdomen cavity of male Wistar rats for duplicating hepatic fibrosis animal model, and the part of them was fed with the above medicine of high, middle, low dose. The quantity of collagen in liver slide was observed. The proliferation of rat hepatic stellate cell (HSC) and the proliferation inhibited by sera contained Chinese medicine of separated rat HSC stimulated with lipopolysaccharide (LPS) were measured with MTT method. Results: Collagen quantity of liver slide in animal of low dose group was significantly less than that of negative medicine control group (F<0. 01), and not significant difference between it and normal control group (F>0. 05). The results showed that the sera contained Chinese medicine obviously inhibited proliferation of separated rat HSC activated with LPS(P<0. 01). Conclusion: The Chinese medicine shows obvious effect against rat experimental hepatic fibrosis in vivo, and the one of mechanisms of the effect is that inhibits proliferation of HSC.

关 键 词：实验研究 益气活血 肝纤维化 肝星状细胞 中药 药理 

分 类 号：R259[医药卫生—中西医结合]