亲和膜用于去除医药及人血清白蛋白溶液中的内毒素(英文)  

Endotoxin Removal in Some Medicines and Human Serum Albumin Solution by Affinity Membranes

作　　者：魏桂林[1] 刘学良[1] 李京华[1] 刘莺[1] 商振华[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116012

出　　处：《色谱》2002年第2期108-114,共7页Chinese Journal of Chromatography

基　　金：SupportedbothbytheNationalNaturalScienceFoundationofChina (NSFC ,2 96 75 0 2 6 )andtheYouthScienceFoun dationofDalianInstituteofChemicalPhysics,TheChineseAcademyofSciences ThisworkearnedtheSecondClassInventionAwardofTheChineseAcadamyofsciencesin 2 0 0 0

摘　　要：以纤维素膜为基质材料 ,制备了 3种用于去除内毒素的亲和膜 ,分别为壳聚糖亲和膜 (KFCC5 17) ,疏水阳离子亲和膜 (KFCG316 )和金属螯合物亲和膜 (KFCG40 2 )。研究了亲和膜对内毒素的吸附容量及在内毒素去除方面的应用 ,结果表明这 3种亲和膜都可以用于多种溶液中内毒素的去除。考察了离子强度、pH值以及流速对去除效果的影响 ,并分析了原因。KFCC5 17,KFCG316 ,KFCG40 2都可用于人血清白蛋白溶液中内毒素的去除 ,KFCC5 17还可用于医药制剂如氢化可的松、葛根素、盐酸丁卡因和右旋糖苷 40葡萄糖注射液中内毒素的去除 ,在所选择的条件下 ,内毒素的去除效率高于 80 % ,有用物质的回收率为 95 %以上。Three kinds of affinity membranes utilized for endotoxin removal, namely, chitosan affinity membrane (KFCC517), hydrophobic and cationic charged membrane (KFCG316) and metal chelate affinity membrane (KFCM402) were prepared by using cellulose membrane as support. The adsorbability and applications of the membranes in endotoxin removal were investigated. The results showed that all of these affinity membranes could be utilized for endotoxin removal in several kinds of solutions. The factors affecting endotoxin removal such as ionic strength, pH value and flow rate were investigated, and the results were discussed. Three cartridges of membranes KFCC517, KFCG316 and KFCM402 were utilized for endotoxin removal in human serum albumin solution. The cartridge of membrane KFCC517 also was utilized in medicine injections, such as hydrocortisone, puerarin, tetracaine hydrochloride and dextran 40 glucose. Under the optimal conditions, the removal efficiencies of endotoxin were over 80% and the recoveries of desirable substances were over 95%.

关 键 词：内毒素 亲和膜 人血清白蛋白 医药制剂 吸附 纤维素膜 

分 类 号：TQ460.6[化学工程—制药化工] R457[医药卫生—治疗学]