半制备型色谱柱与色谱饼性能的比较  

Comparison of the Separation Performances between Semi-Preparative Chromatographic Column and Chromatographic Pie

作　　者：李翔[1] 张养军[1] 耿信笃[1] 

机构地区：[1]西北大学现代分离科学研究所,陕西西安710069

出　　处：《色谱》2002年第2期133-136,共4页Chinese Journal of Chromatography

基　　金：国家自然科学基金资助项目 (批准号 :2 96 70 17;39880 0 0 3)

摘　　要：通过比较具有相同柱几何体积的半制备色谱柱和色谱饼的性能 ,发现在同样的色谱条件下 ,用液相色谱方法分离生物大分子时 ,在分离效率和蛋白活性回收方面 ,色谱饼和色谱柱的差异不大 ;由于色谱饼中的填料较色谱柱中的装填更为紧密和均匀 ,当流动相流速增加时 ,色谱饼不仅反向压力上升慢 ,而且质量和体积负载也比色谱柱高。该结果表明使用色谱饼的色谱分离不仅具有可与高效液相色谱 (HPLC)媲美的分离效率 ,而且还具有中、低压色谱分离的高柱负载 ,这有利于蛋白保持高的生物活性 ,为色谱饼的工业化应用奠定了基础。By comparing the resolution and recoveries of proteins between semi-preparative chromatographic column and chromatographic pie (pie-like column) having the same geometric volume under the same chromatographic conditions, the result from the former was found to have the almost same as that form the latter. However, the back pressure of the chromatographic pie rose slower than that of the chromatographic column with the increase of flow rate. Furthermore, the mass and volume loads for the chromatographic pie were higher than those for the chromatographic column. The results indicated that the chromatographic pie had more advantages than the chromatographic column in their performances, and this lay a foundation of the applications of the chromatographic pie in industry.

关 键 词：液相色谱 半制备色谱柱 半制备色谱饼 性能 生物大分子 分离 

分 类 号：O657.72[理学—分析化学] Q503[理学—化学]