葡萄糖及其衍生物的直接紫外检测毛细管区带电泳研究  

Direct UV Detection of Glucose and Its Derivatives in Capillary Zone Electrophoresis

作　　者：许峰[1] 祝文君[1] 毛秀丽[1] 戴忠鹏[1] 张青[1] 杜昱光[1] 梁鑫淼[1] 林炳承[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《色谱》2002年第2期156-158,共3页Chinese Journal of Chromatography

基　　金：中国科学院创新基金 (KSCX 2 3 0 2 0 2 );国家自然科学重点基金 ( 2 0 0 35 0 10 )

摘　　要：发展了在 195nm波长下直接检测葡萄糖及其衍生物的毛细管区带电泳方法。在未涂渍石英毛细管中 ,以 5 0mmol/LNa2 HPO4 5 0mmol/LNaH2 PO4 为缓冲液体系 (接近生理条件 pH 7 4) ,分离了葡萄糖及其衍生物 (葡萄糖胺、N 乙酰葡萄糖胺和葡萄糖酸钠 )。在各自相应的浓度范围内 ,峰面积与样品浓度之间呈现良好的线性关系。方法简单、快速、重复性好 ,为研究葡萄糖及其衍生物与凝集素的相互作用奠定了分离检测的基础。UV labeling detection has been commonly used to determine the association constants between lectins and saccharides, but the interaction is always between the labeled carbohydrates, rather than the truly underivatized carbohydrates, and lectins. In order to directly detect saccharides during the study on the interaction of glucose and its derivatives with lectins (e.g., concanavalin A), a capillary zone electrophoretic method with detection at a wavelength of 195 nm has been developed. The influences of various separation conditions including buffer concentration, pH and voltage were investigated. By using an uncoated silica capillary (50 μm i.d., 375 μm o.d., 48.5 cm of total length, and 44.0 cm to the detector) and 50 mmol/L Na_2HPO_4-50 mmol/L NaH_2PO_4 solution (near to the physiological pH of 7.4) as buffer, the underivatized sugars, including glucosamine, N -acetylglucosamine, glucose, and sodium gluconate, were sufficiently separated within 11 min at an applied voltage of 10 kV. On-column UV monitoring allowed the detection of these compounds at less than 4 mmol/L level, and quantification by the peak area method allowed reproducible determination of them at least at their respective concentration ranges. The method is characterized by its simplicity, rapidity, and reproducibility, and should be useful for the analysis of the interaction of glucose and its derivatives with lectins.

关 键 词：毛细管区 带电泳 直接紫外检测 葡萄糖 衍生物 分析 分离 

分 类 号：O629.112[理学—有机化学] O657.8[理学—化学]