神经营养因子对p75NTR诱导哺乳动物神经细胞凋亡的影响  

EFFECT OF NEUROTROPHIC FACTOR ON APOPTOSIS OF MAMMAL NEURONS INDUCED BY p75NTR

作　　者：明洪[1] 黄秉仁[2] 张闻[1] 曾惠芳[1] 李京昆[1] 

机构地区：[1]昆明医学院生物学教研室，昆明 650031 [2]中国协和医科大学基础医学院，北京 100005

出　　处：《中国组织化学与细胞化学杂志》2002年第1期55-58,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的　研究 R2 L 1细胞的凋亡以及神经营养因子对凋亡的影响。方法　形态学观察、细胞活性测定和流式细胞仪分析。结果　 R2 L 1细胞去血清培养 12小时即发生凋亡 ,2 4小时后大部分细胞凋亡 ,48小时后细胞基本上都死亡。结论　 NGF、 NT- 3和抗 p75 NTR抗体能抑制 R2 L 1细胞在去血清培养后发生的凋亡 ,而? Objective In the experiment, R2L1 cells began to undergo apoptosis after being deprived of serum for 12 hours. Method Most cells underwent apoptosis at 12 24hours, and more than 95% were dead by 48 hours. Conclusion The study also showed that NGF?NT 3 and antibodies against p75NTR could inhibit the apoptosis of R2L1 cells deprived of serum, but BDNF could not.\;〔

关 键 词：神经营养因子 P75NTR 哺乳动物 神经细胞凋亡 Trk受体家族 大鼠 

分 类 号：Q421[生物学—神经生物学] R338.1[生物学—生理学]