镍邻菲绕啉对DNA固定作用的原子力显微镜表征  

The AFM Characterization of Ni(phen)_3(2+)Fixing Effects on DNA

作　　者：于力华[1] 李壮[1] 吴爱国[1] 王宏达[1] 索全伶[2] 毕晓辉[3] 黄百渠[3] 

机构地区：[1]中国科学院长春应用化学研究所电分析化学开放研究实验室,长春130022 [2]内蒙古工业大学化工学院化学系,呼和浩特010064 [3]东北师范大学遗传与细胞研究所,长春130024

出　　处：《高等学校化学学报》2002年第1期46-48,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :30 0 70 417)资助

摘　　要：In this paper, the fixing and stretching effect of Ni(phen) 2+ 3 with different concentrations on DNA had been studied by Tapping mode AFM. Under an ambient condition, the high resolution DNA images were obtained, the average height, width and length of well spread DNA molecules were measured. The results showed that because of the variations of ionic concentration, the density and topography of DNA molecules on substrate had a great difference. The AFM and gel electrophoresis results also showed that, under our experimental condition, DNA molecules kept intact, Ni(phen) 2+ 3 did not catalyze the cleavage activity of EcoRI, therefore, Ni(phen) 2+ 3 would be used to make high resolution physical mapping of DNA by AFM.In this paper, the fixing and stretching effect of Ni(phen) 2+ 3 with different concentrations on DNA had been studied by Tapping mode AFM. Under an ambient condition, the high resolution DNA images were obtained, the average height, width and length of well spread DNA molecules were measured. The results showed that because of the variations of ionic concentration, the density and topography of DNA molecules on substrate had a great difference. The AFM and gel electrophoresis results also showed that, under our experimental condition, DNA molecules kept intact, Ni(phen) 2+ 3 did not catalyze the cleavage activity of EcoRI, therefore, Ni(phen) 2+ 3 would be used to make high resolution physical mapping of DNA by AFM.

关 键 词：原子力显微镜 脱氧核糖核酸 DNA 镍邻菲绕啉 凝胶电泳 固定作用 表征 

分 类 号：Q523[生物学—生物化学] Q503