鼠脑尾核和海马区微透析液中儿茶酚胺类神经递质的高效液相色谱分析  

High Performance Liquid Chromatographic Analysis of Catecholaminein the Microdialysate from Rat Brain

作　　者：常理文[1] 徐桂芸[1] 韩慧婉[1] 赵卓[1] 常溪燕[2] 

机构地区：[1]中国科学院化学研究所分子科学中心,北京100080 [2]北京石油化工科学研究院,北京100083

出　　处：《高等学校化学学报》2002年第4期552-554,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:29775027);中国科学院基础性研究重大项目(批准号:KJ-951-A1-507-03)资助.

摘　　要：采用反相液相色谱分离－脉冲安培检测和微透析取样技术,测定了取自鼠脑尾核和海马区微透析液中儿茶酚胺类神经递质的含量．对不同神经活动状态下取得微透析液中肾上腺素含量作了比较,将该法用于测定肾上腺素,灵敏度为２５μｇ/Ｌ．A method for determination of catecholamine and related metabolite in the microdialysate fromrat brain by HPLC With pulsed amperometric detection was described. The optimum condition of chro-matographic separation and pulsed amperometri detection were studied. Sampling was carried out by mi-crodialysis technique from rat brain. A comparison of epinephrine(E) content in microdialysates from ratbrain at various event was made. The detection limit of the method is 25 μg/L for epinephrine.

关 键 词：神经递质 微透析取样 脉冲安培检测 高效液相色谱 几茶酚胺 海马区 脑导核区 

分 类 号：Q42[生物学—神经生物学]