加替沙星铽敏化化学发光与应用  

Terbium Sensitized Chemiluminescence of Gatifloxacin and Its Application

作　　者：连宁[1] 赵慧春[1] 孙春燕[1] 金林培[1] 张仲伦[2] 郑雁珍[2] 

机构地区：[1]北京师范大学化学系,北京100875 [2]中国科学院生物物理研究所,北京100101

出　　处：《高等学校化学学报》2002年第4期564-566,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:29971005);国家重点基础研究发展规划项目(批准号:G1998061308)及北京市自然科学基金资助.

摘　　要：加替沙星(Gatifloxacin,GFLX),卜环丙基－6－氟－7－(3－甲基－1赈嗓基)－8－甲氧基－1,个二氢－个氧－隆琳－3－竣酸1/2水合物是一种新型的第三代氟哆诺酮抗菌药物,与现有的氟喳诺酮类药物相比,A selective and sensitbe sensitized chemiluminescence (CL) method for the determination ofGatifloxacin(GFLX) is described. The method is based on Tb3+ sensitized chemiluminescence of the Tb3+-GFLX-Ce(Ⅳ)-SO3_3^(2-) system since CL for the binary Ce(Ⅳ)-SO_3^(2-) system is weak, while CL is enhancedwhen Tb3+-GFLX is added to the Ce(Ⅳ)-SO_3^(2-) system. The possible mechanism of the CL reaction maybe that Ce(Ⅳ) can oxidize HSO_3^- to a hydrogen-sulfite radical HSO3, then HSO3 radicals produce S2O_6^(2-),S2O_6^(2-) will form the excited intermediate product SO2. The energy of SO2 is mainly transferred to Tb3+-GFLX, and the CL spectrum shows a characteristic emission of Tb3+. The re1ationship between the CL in-tensity and the concentration of GFLX is linear in the range of 2.0 × 10-8-1.0×10-6 mol/L, and the de-tection limit is 3. 5 × 10-9 mol/L. The relative standard deviation(RSD) for 11 independent determinationsof 1.0 X 10-7 mol/L GFLX was 3.2%. Common excipient starch was used in pharmaceutical preparationand most of metal ions do not interfere with the determination of GFLX. GFLX in urine can be analyzeddirectly after appropriate dilution of the samples, and the analytical result is satisfactory.

关 键 词：加替沙星 铽 化学反光 能量转移 流动注射 抗菌药物 药物分析 

分 类 号：R978.1[医药卫生—药品] R917[医药卫生—药学]