噬菌体表面展示人干扰素-α 2b  

Phage display of human interferon alpha 2b

作　　者：吴晓兰[1] 潘卫[1] 马仲才[1] 陈秋莉[1] 武文斌[1] 曹明媚[1] 戚中田[1] 

机构地区：[1]第二军医大学基础医学部微生物学教研室,上海200433

出　　处：《第二军医大学学报》2002年第4期403-406,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目(39830330;C03011403).

摘　　要：目的：建立一种研究人干扰素-α结构与功能的新平台。方法：利用噬菌体表面展示（phage display）技术表达人于扰素-α2b（hIFN-αa2b）基因。结果：生物活性测定表明，3×108TU重组噬菌体干扰素的抗病毒活性与SIU基因工程IFN-α2a的活性相当，并能被 IFN-α单抗中和。结论：hlFN-α 2b在噬菌体表面能正确折叠和功能性表达，即成功地建立了噬菌体展示外源肽（hIFN-α）的技术平台。Objective: To develop a new platform for studying the structure and function of human interferon alpha(hIFN-α). Methods: Human interferon alpha 2b (hIFN-α2b) gene was amplified by PCR and cloned into phage display vectorpCANTAB5X. So hIFN-α 2b was displayed on the surface of phage, which was termed as hIFN-α 2b phage, in a form fusedwith gene Ⅲ protein (g3p). Results: It was shown that 3×10~8 TU hIFN-α 2b phage possessed antiviral biological activity onVSV challenged WISH cells, being comparable to that of 5 IU hlFN-α 2a, and this activity was neutralized by hIFN-α specificantibodies. Conclusion: These results demonstrate that hlFN-α 2b can be displayed on phage in a correctly folded and func-tionally active form, this technique platform is successfully constructed and it is applicable in detecting structure and functionof hIFN-α.

关 键 词：人干扰素-α 抗病毒活性 噬菌体表面展示 

分 类 号：Q782[生物学—分子生物学]