葡萄扇叶病毒引起的寄主细胞病变研究  

ULTRASTRUCTURAL ALTERATION OF PLANTS INFECTED WITH Grapevine fanleaf virus

作　　者：李红叶[1] 洪健[2] 周雪平[1] 陈力耕[1] 

机构地区：[1]浙江大学农业与生物技术学院 [2]浙江大学分析测试中心,杭州310029

出　　处：《植物病理学报》2002年第1期65-79,共15页Acta Phytopathologica Sinica

基　　金：浙江省自然科学基金 (3994 86 )

摘　　要：电镜观察了葡萄扇叶病毒杭州分离物 (Grapevine fanleaf virus Hangzhou isolate,GFLV- H)侵染苋色藜 (Chenopodium amaranticolor)和昆诺藜 (C.quinoa)的细胞超微结构变化。病毒粒子存在于叶肉组织薄壁细胞的细胞质内 ,常成单纵列排列在小管结构中 ,小管形成聚集体。在系统感染的昆诺藜细胞中位于管状结构中的病毒粒子穿过胞间连丝。 2种感病寄主细胞质内膜结构增生 ,在核周围形成含细纤维状物质的小囊泡。液泡边缘存在少许小泡 ,有多泡体和髓鞘样结构伸入液泡 。The ultrastructural alteration of Chenopodium amaranticolor and Chenopodium quinoa infected with Grapevine fanleaf virus Hangzhou isolate (GFLV H) was studied by transmission electron microscope. Isometric virus particles were found in the cytoplasm of infected parenchyma cells of leaf tissue. The virus particles often lined up in aggregated tubules. In systemically infected C. quinoa virus particles are aligned in tubular structure protruding through the cell wall within plasmodesmata. Membranous proliferation and fibril containing vesicles developed in the cytoplasm, usually adjacent to the nucleus in both host plants. Some multivesicular bodies and myeloid bodies protruding into the vacuole and virus specific vesicles associated with the tonoplast were also observed. The cellular organization, such as chloroplast and mitochondria, were often affected.

关 键 词：寄主 细胞病变 葡萄 扇叶病毒 细胞病理学 电子显微镜 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]