苹果和梨树茎尖培养结合热处理脱病毒研究  

Studies on Elimination of Apple and Pear Virus by Combining Tissue Culture of Top-Stem with Heat Therapy

作　　者：董雅凤[1] 张尊平[1] 张少瑜[1] 洪霓[2] 于济民[1] 

机构地区：[1]中国农业科学院果树研究所,辽宁兴城125100 [2]华中农业大学,武汉430070

出　　处：《北方果树》2002年第2期9-11,共3页Northern Fruits

摘　　要：1995～2000年,以18个苹果品种和15梨品种为试材,以茎尖培养结合热处理进行脱毒研究。用酶联免疫吸附法检测结果表明,苹果茎沟病毒和褪绿叶斑病毒脱毒率可分别达到75%以上和100%。现已获得16个苹果品种和6个梨品种的无病毒母本树。During1995～2000,18apple varieties and15pear varieties were used for the study on elimination by combine the top -stem tissue culture with heat therapy.The examining result by ELISA showed that the elimination rates of ASGV(apple stem grooving virus)and CLSV(chlorotic leaf spot virus)were over75%and100%.Virus-free mother trees of16apple varieties and6pear varieties had been obtained in the study.

关 键 词：苹果 梨 茎尖培养 热处理 脱病毒 

分 类 号：S436.611[农业科学—农业昆虫与害虫防治]