米非司酮对人早孕期蜕膜细胞凋亡及Fas/FasL的影响  

THE EFFECT OF MIFEPRISTONE ON APOPTOSIS AND APOPTOTIC GENE Fas/FasL EXPRESSION OF DECIDUA IN HUMAN EARLY GESTATIONAL PERIOD

作　　者：丁峰[1] 傅国强[2] 邢福祺[1] 陈士岭[1] 刘以训[2] 

机构地区：[1]广州第一军医大学南方医院 [2]中国科学院动物研究所计划生育生殖生物学国家重点实验室

出　　处：《解放军医学杂志》2002年第4期362-363,共2页Medical Journal of Chinese People's Liberation Army

基　　金：中国科学院动物研究所计划生育生殖生物学国家重点实验室WHO/RockeFeller基金会及国家计生委 (编号 9738)和广东省自然科学基金 (编号 970 343)

摘　　要：为探讨米非司酮对人早孕期蜕膜组织细胞凋亡及相关基因Fas、FasL的影响 ,应用TUNEL法检测凋亡的发生 ,原位杂交和免疫组化法检测Fas、FasL的表达和相互关系。结果发现 :①正常早孕 4 0 + 天蜕膜组织细胞大量凋亡 ,Fas、FasLmRNA及蛋白有较强表达。②正常早孕 5 0 + 天 ,凋亡细胞明显减少 ,Fas、FasL的表达明显减弱。③早孕 5 0 + 天服用米非司酮 ,蜕膜组织大量凋亡 ,且Fas、FasL的表达较正常早孕 5 0 + 天增强。提示 ,米非司酮抗早孕的机制之一可能是通过促进蜕膜组织的异常凋亡实现的 。The present study was designed to investigate the effects of mifepristone on apoptosis of the decidual tissue cells and apoptotic correlated gene Fas/FasL expression in human early gestational period. The extent of DNA fragmentation of apoptotic cells were assessed by using an in situ terminal transferase reaction to label free 3′ ends of the DNA.The expression of Fas, FasL in the deciduas was examined by in situ hybridization with cDNA probe and immunohistochemical staining with polyclonal antibodies, respectively. The results showed that there were evident apoptosis in decidual tissues at about 40 days of gestation. Fas and FasL were strongly expressed in these decidual tissues. At about 50 days of gestation, there were a few apoptotic cells in the decidual tissues. Expression of Fas, FasL decreased at the same time. More apoptotic cells were observed in decidual tissues after adiministration of Mifepristone (RU486) in about 50 days of gestation. Fas, FasL mRNA and protein were up regulated after administration of Mifepristone. The results suggested that the induction of apoptosis by Mifepristone in decidual tissues might be one of the major mechanisms with regards to contraceptive and antigestational effects of mifepristone. Fas pathway might participate in the process of decidua apoptosis induced by RU486.

关 键 词：子宫蜕膜 细胞凋亡 米非司酮 Fas FASL 药物流产 

分 类 号：R169.42[医药卫生—公共卫生与预防医学]