激素性股骨头坏死动物模型的诱导及观察  

Inducement and Observation of Animal Model of Steroid-treated Osteonecrosis

作　　者：黄健[1] 郭文通[1] 李文琪[1] 刘万林[1] 

机构地区：[1]内蒙古医学院第二附属医院骨科,内蒙古呼和浩特010030

出　　处：《内蒙古医学杂志》2002年第2期103-105,共3页Inner Mongolia Medical Journal

摘　　要：目的：通过马血清、激素联合作用于实验家兔　，制备典型骨坏死动物模型，进一步探究激素性股骨头坏死的发病机理。方法：选新西兰白　兔６０只，随机分成４组。Ａ组：２０只，经静脉２次单纯注射马血清（１０ｍｌ／ｋｇ／次），间隔３周。Ｂ　组：１５只，连续３日腹腔内注射甲基强的松龙（每日４５ｍｇ／ｋｇ），Ｃ组：２０只，先静脉２次注射马　血清（１０ｍｌ／ｋｇ／次），间隔２周。第２次注射马血清后２周，连续３日腹腔内注射甲基强的松龙（　每日４５ｍｇ／ｋｇ）。Ｄ组：５只，对照组。分别于最后１次给药后１、２、３、５、７周处死取材。标本　常规组织病理学检查，并进行动脉墨汁灌注观察。结果：在马血清和激素协同作用的Ｃ组中　，股骨头内小动脉损害严重，血栓形成广泛，软骨下灌注血管数量减少明显，共有７４％的标　本发生骨坏死。结论：激素性股骨头坏死是一个复杂的病理生理过程，是由多因素共同作用的结果。Objective:Make the typical animal m odel of osteonecrosis,by employing a combined action of horse serum and corticos teroid in experimental rabbits,to further investigate the pathogenesis of steroi d-induced osteonecrosis.Method:Group A(20) was received 10ml/kg of horse serum i ntravenously two times at three week intervals.Group B(15)was received an intrap eritoneal injection of 45mg/kg/day of methylprednisolone acetate for three conse cutive days.GroupC(20)was injected 10ml/kg of horse serum intravenously twice.Tw o weeks after the second serum injection,45mg/kg/day of methylprednisolone acetat e was injected intraperitoneally for three consecutive days.Group D(5)was used a s the control.These rabbits were killed respectively one,two,three,five,seven we eks after their final treatment,and were examined histologically.Intraartery ink perfution were observed in some rabbits at the different time of treatment.Resu lts:The findings of artery perfusion and hitological studying showed that vascul ar wall of the arterioles was severely impaired,multible thrombi came into being ,the number of perfusion vascular decreased significantly and 74% rabbits develo ped osteonecrosis in group c.Conclusion:Steroid-induced osteonecrosis was a comp licated pathophysiologic process,and a common result of multiple factors.

关 键 词：激素性股骨头坏死 动物模型 诱导 缺血性骨坏死 发病机理 

分 类 号：R681.8[医药卫生—骨科学]