白介素8及其受体CXCR2在银屑病角质形成细胞中的表达  

Expression of IL-8 and CXCR2 on Keratinocytes from Psoriatic Lesions

作　　者：唐玲[1] 顾军[1] 陶苏江[1] 郑茂荣[1] 于益芝[2] 王闻雅[2] 刘书逊[2] 曹雪涛[2] 

机构地区：[1]上海第二军医大学附属长海医院皮肤科 [2]第二军医大学免疫学研究所

出　　处：《中华皮肤科杂志》2002年第2期105-107,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(39825123;39970689);上海市科技启明星计划资助(99QB14047)

摘　　要：目的观察白介素8(IL-8)及其受体CXCR2在银屑病角质形成细胞中的表达及在银屑病的临床及病理表现中的作用。方法培养银屑病患者皮损处角质形成细胞,通过微孔小室实验检测其上清液的趋化功能,通过酶联免疫吸附法(ELISA)检测上清液中IL-8的表达,通过流式细胞仪分析银屑病患者皮损处角质形成细胞的趋化因子受体CXCR2的表达。结果银屑病患者皮损处角质形成细胞分泌上清液对中性粒细胞的趋化能力明显强于正常对照组,其分泌的IL-8水平也高于正常人,皮损处角质形成细胞CXCR2的表达也明显强于正常对照组。结论银屑病患者皮损局部表现出的角质形成细胞高度增殖与角质形成细胞高分泌、高表达具有促增殖作用的IL-8及其受体CXCR2有关,同时皮损局部大量的炎性细胞的浸润部分可能是由于角质形成细胞高分泌具有趋化能力的IL-8,它们可能在银屑病的发生、发展中起着重要作用。Objective To investigate the expression of IL-8 and CXCR2 on keratinocytes from psoriatic lesions and their roles on clinical and pathologic manifestations. Methods The chemotaxis of psoriatic lesional keratinocytes was detected by micropore loculus test. The concentration of IL-8 was determined in the cultured supernatants of psoriatic keratinocytes by ELISA. The expression of CXCR2 on keratinocytes from affected skin was tested by flow cytometry. Results The chemotaxis for neutrophils by the cultured supernatants of psoriatic lesional keratinocytes was significantly stronger than that by controls. The concentration of IL-8 in the cultured supernatants of psoriatic lesional keratinocytes was also increased. The expression of CXCR2 on psoriatic keratinocytes was significantly increased. Conclusions The psoriatic epidermal hyperproliferation may be correlated with up regulation of IL-8 production and CXCR2 expression on psoriatic keratinocytes. At the same time, the psoriatic inflammation may be partly related to the increase of secretion of IL-8, which has chemotactic capacity, by keratinocytes. IL-8 and CXCR2 may be involved in the pathogenesis of psoriasis.

关 键 词：银屑病 白细胞介素8 CXCR2 角质形成细胞 皮损 趋化因子 酶联免疫吸附法 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]