芦荟素、肉桂酸、苦参碱对酪氨酸酶的抑制作用  

Monomers of Aloin,Cinnamic Acid and Sophocarpidine Acting as Potent Inhibitors of Tyrosinase

作　　者：谭城[1] 朱文元[1] 鲁严[1] 刘栋[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,210029

出　　处：《中华皮肤科杂志》2002年第2期134-136,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助课题(39870701和39570658)

摘　　要：目的研究芦荟素、肉桂酸、苦参碱等15种单体化学物对酪氨酸酶活性的影响,判定其抑制类型,从而为色素增加性皮肤病和外用皮肤脱色剂提供药物。方法酪氨酸酶多巴速率氧化法体外测定药物干预前后酪氨酸酶活性,求出酪氨酸酶抑制率,并分别作出相应Lineweaver-Burk曲线,推断其抑制类型。结果15种中药单体化学物中,芦荟素、肉桂酸和苦参碱能显著抑制酪氨酸酶活性,其中芦荟素(2mmol/L)、苦参碱(2mmol/L、0.5mmol/L)酪氨酸酶抑制率高于氢醌(0.5mmol/L)(P<0.05)。结论芦荟素、肉桂酸、苦参碱等均可下调酪氨酸酶活性。苦参碱属于酪氨酸酶非竞争性抑制剂,芦荟素、肉桂酸属混合型抑制剂。Objective To evaluate the effects of monomers of aloin, cinnamic acid and sophocarpidine on the acitivity of tyrosinase,so as to provide depigmenting agents in the treatment of hyperpigmentation disorders and cosmetics additives as well. Methods Tyrosinase activity was estimated by measuring the oxidation rate of DL dopa. The inhibitory pattern of each monomers was determined according to their Lineweaver Burk curve as compared to controls. Results Aloin,cinnamic acid and sophocarpidine down regulated the activity of tyrosinase. The inhibitory rates were significantly higher in cinnamic acid group(2 mmol/L, 0.5 mmol/L), aloin group (2 mmol/L)and sophocarpidine group than those in hydroquinone group (0.5 mmol/L)(P< 0.05). Conclusion Tyrosinase activity can be greatly inhibited by cinnamic acid, aloin and sophocarpidine, of which sophocarpidine functions as an uncompetitive inhibitor, while aloin and cinnamic acid as mixed type inhibitors of tyrosinase.

关 键 词：芦荟素 桂皮酸 苦参碱 酷氨酸酶 皮肤脱色剂 皮肤黑素 

分 类 号：R285.5[医药卫生—中药学]