灌喂必需氨基酸模式溶液对草鱼全鱼和肌肉、肝胰脏蛋白质合成代谢的影响  

Effects of infusing EAA pattern solution on protein synthesis in whole body, muscle and hepatopancreas of Ctenopharyngodon idellus

作　　者：罗莉[1] 叶元土[1] 林仕梅[1] 

机构地区：[1]西南农业大学水产系,重庆400716

出　　处：《水产学报》2002年第1期73-78,共6页Journal of Fisheries of China

基　　金：重庆市科委攻关项目 (97-4 767)

摘　　要：采用大剂量食道灌喂DL [4 3 H] Phe ,研究不同EAA模式下 ,2 0～ 30g体重草鱼种的全鱼和肌肉、肝胰脏的蛋白质合成代谢。结果表明 :①分别缺乏Lys、Met、Trp、Arg的 4种极端不平衡EAA模式 ,同草鱼肌肉EAA模式即相对平衡的基础模式相比 ,肌肉、肝胰脏及全鱼的蛋白质合成速率 (FSR)均显著降低 ,其降低程度高低依次为Met,Lys ,Arg ,Trp ;②肝胰脏、肌肉的蛋白质合成代谢与整体蛋白质合成代谢对EAA模式的改变产生相同的变化趋势 ,并且三者蛋白质合成代谢速率为肝胰脏 >全鱼 >肌肉 ;③EAA模式的Lys、Met、Trp、Arg4因素中 ,对肌肉、肝胰脏蛋白质合成速率影响最大的是Met ,其余依次为Lys ,Arg ,Trp。The experiment adopts oesophageal infusion with large dose DL Phe to research protein synthesis metabolism in whole body, muscle and hepatopancreas of 20～30g grass carp in different essential amino acid(EAA) patterns. The results show that: 1.Four imbalance EAA patterns, respectively short of Lys?Met?Trp?Arg, compared with EAA pattern of muscle, result in remarkable fractional protein synthesis rate (FSR) decrease of muscle, hepatopancreas and whole body, which shows the decreasing by Met>Lys>Arg>Trp. 2.Following the variation of EAA pattern, FSR of muscle, hepatopancreas and whole body has a similar change, and shows FSR by: hepatopancreas>whole body>muscle. 3.In Met,Lys,Arg and Trp four factors of EAA pattern, from Met to Trp have gradually and orderly weakened impact on protein synthesis in muscle and hepatopancreas of grass carp, in other words, Met has the largest impact.

关 键 词：草鱼 蛋白质周转代谢 肌肉 肝胰脏 必需氨基酸模式 鱼类养殖 

分 类 号：S965.112[农业科学—水产养殖]