雷震子护康胶囊制剂抗氧化和对DNA损伤保护作用研究  

Effects of Lei Zhen Zi Health-Care Capsule on Antioxidation and Preventing DNA Damage Caused by Hydroxyl Radical

作　　者：翟万银[1] 朱振勤[1] 陈季武[1] 王云丹[1] 赵子寅[1] 胡天喜[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《发光学报》2002年第2期191-196,共6页Chinese Journal of Luminescence

摘　　要：雷震子护康胶囊以低聚原花青素、灵芝多糖、绞股蓝总皂甙及一定量的维生素C等为主要成分而制成 ,有多种保健功能。为研究该复方制剂的抗氧化作用 ,采用七种化学发光体系和一种比色体系研究了雷震子直接清除活性氧、活性氮和对·OH引起的DNA损伤的保护作用。结果表明雷震子能有效清除O2·、·OH、H2 O2 、ONOO-和全血嗜中性白细胞“呼吸爆发”产生的活性氧 ,抑制脂质过氧化 ,保护DNA免受·OH的氧化损伤。这些结果提示 ,雷震子是一种有效的抗氧化复方制剂 。As a nutrient supplement, Lei Zhen Zi Health-Care Capsule (Lei Zhen Zi) shows good effects on immune regulation and anti-aging. Its main components are procyanidolic oligomers, Ganoderma lucidum polysaccharides, gypenoside and vitamin C. Although some physiological effects of each component have been studied, the ability of Lei Zhen Zi as a combination form of nutrient supplement to scavenge directly free radicals, reactive oxygen species and reactive nitrogen species has never been investigated. In the present study, seven chemiluminescence systems and one colorimetric system, well established in our labora- tory, were used to elucidate the effects of Lei Zhen Zi on antioxidation and preventing DNA damage caused by ·OH oxidation. The experiments revealed that Lei Zhen Zi could effectively scavenge O 2 · produced in both xanthine-xanthineoxidase chemiluminescence system and pyrgallic acid auto-oxidation chemiluminescence system, but the 50% inhibition concentration (IC 50) in the former (0.4μg/mL) was much lower than that in the later (50μg/mL). The reason for this phenomenon may be that in the xanthine-xanthineoxidase chemiluminescence system, Lei Zhen Zi can not only directly scavenge O 2 ·, but also inhibit the activity of xanthineoxidase and reduce the production of O 2 ·, whereas in the pyrgallic acid auto-oxidation chemiluminescence system, Lei Zhen Zi acts only as an O 2 · scavenger. The experiments also showed that Lei Zhen Zi could effectively scavenge ·OH produced in CuSO 4-Vit.C chemiluminescence system (IC 50=100μg/mL), H 2O 2 in H 2O 2-luminol-OH -system (IC 50=0.5μg/mL), ONOO - in ONOO --luminol chemiluminescence system (IC 50=50μg/mL) and the reactive oxygen species produced in whole blood phagocytes chemiluminescence system (IC 50=12μg/mL). The DNA-protecting experiments showed that Lei Zhen Zi could effectively prevent DNA damage caused by ·OH oxidation through direct scavenge of ·OH. Above results indic

关 键 词：雷震子 活性氧 活性氮 DNA损伤 抗氧化剂 损伤保护 复方制剂 雷震子护康胶囊 

分 类 号：R994.5[医药卫生—毒理学] R977.9[医药卫生—药学]