鸡胚细胞内微量元素分析  

Analysis of trace elements in chicken embryo cells

作　　者：仇志军[1] 王基庆[1] 郭盘林[1] 李晓林[1] 朱节清[1] 陆荣荣[1] 

机构地区：[1]中国科学院上海原子核研究所,上海201800

出　　处：《核技术》2002年第4期299-304,共6页Nuclear Techniques

基　　金：中国科学院九五重大基础研究项目 (2 10 3975 3)

摘　　要：本文用高分辨率、高灵敏度的扫描质子微探针 (SPM )对鸡胚前脑神经元细胞和骨骼肌肌管细胞中的微量元素进行分析 ,研究了这两种不同细胞对Zn离子的吸收情况、细胞中元素的相关关系以及微量元素在细胞内的分布情况。结果发现 ,鸡胚前脑神经元细胞对Zn离子的吸收能力明显大于骨骼肌肌管细胞 ;并且细胞中Cr、Fe、Ni等微量元素的浓度含量明显偏高 ;细胞中S -Zn、Fe -Zn等元素呈正相关关系 ,而P -Ni、Cr-Fe则呈负相关关系。从细胞的元素分布图上还发现不同元素在细胞各个组成部分的含量是不同的 ,如P、S。A scanning proton microprobe (SPM) with high resolution and high sensitivity was applied to analyze trace elements in chicken embryo forebrain neuron cell and skeletal muscle myotube cell. The absorption of the two different cells to zinc ions, correlation of elements and trace elemental distributions in the cells were studied. The results indicate that the absorptive capacity of the chicken embryo forebrain neuron cell to zinc ions is larger than that of the chicken embryo skeletal muscle myotube cell, and the concentrations of intracellular trace elements such as Cr, Fe, Ni are explicitly higher. The correlations of elements such as S and Zn or Fe and Zn are positive, but the correlations of P and Ni or Cr and Fe are negative. From the maps of cellular elemental distribution the contents of the different elements are different in the intracellular parts, for example, the contents of the elements phosphorus, sulfur, potassium in the cell membranes are higher than that in the cells.

关 键 词：鸡 胚细胞 微量元素 扫描质子微探针 输运 跨膜效应 生物物理机制 

分 类 号：S852.2[农业科学—基础兽医学] TL99[农业科学—兽医学]