电离辐射质粒pUC18 DNA结构变化的傅里叶变换拉曼光谱分析  

Structural Changes of pUC18 DNA Irradiated by Ionizing Radiation Analysed by FT-Raman Spectroscopy

作　　者：王杰芳[1] 王进[2] 杨柳 刁振奇[1] 李富广[3] 

机构地区：[1]河南医科大学物理教研室,河南郑州450052 [2]河南医科大学微生物学与免疫学教研室,河南郑州450052 [3]焦作市教育学院计算机系,河南焦作454152

出　　处：《光谱学与光谱分析》2002年第2期245-247,共3页Spectroscopy and Spectral Analysis

基　　金：河南省教委科研基金资助项目

摘　　要：采用傅里叶变换拉曼光谱研究了质粒 pUC18DNA经过γ射线电离辐射以后结构的变化。其脱氧核糖 磷酸主链中各振动模式产生的谱带强度有所增加 ,包括 884cm-1处的脱氧核糖 磷酸主链振动谱带、146 0cm-1处的 CH2 弯曲振动谱带和 2 939cm-1处的C—H伸缩振动谱带。碱基杂环弯曲振动谱带 187cm-1位移到 2 0 8cm-1处 ,强度明显减弱。在 32 98cm-1处出现了一个源于N—H伸缩振动的新谱带。此结果表明碱基受损 ,主链断裂 ,超螺旋结构部分解旋。Structrual changes of pUC18 DNA irradiated by gamma-ray ionizing radiation were studied by Fourier- transform-Raman spectroscopy. Intensity of the lines of all vibrational modes in the deoxyribose-phosphate chain increases, including the fine of the deoxyribosephosphate chain at 884 cm(-1) the line of deformation vibration of -CH2 at 1 460 cm(-1) and the line of stretching vibration of C-H at 2 939 cm(-1). The band of the deformation vibration of the bases ring at 187 cm(-1) shifts to the line at 208 cm(-1), its intensity decreases evidently. A new band appears at 3 298 cm (-1), assignment of the N-H stretching vibration. The results show bases damaging, strandline breaking, supercoiled structure relaxing partly.

关 键 词：电离辐射 质粒pUC18DNA 结构变化 傅里叶变换拉曼光谱 消毒 灭菌 

分 类 号：Q691[生物学—生物物理学] R187[医药卫生—流行病学]